• single-molecule spectroscopy

    Single-Molecule Spectroscopy

    Using fluorescence to observe protein dynamics.



    Breaking the concentration barrier using smFRET in conjunction with Zero-Mode Waveguides.

  • ZMW mM concentration

    Single-Molecule Resolution at [mM] Concentration

    Direct observation of individual cAMP or cGMP binding dynamics.

  • Structure-Dynamics Model

    Structure-Dynamics Modeling

    Mechanism for initial stages of cyclic nucleotide regulation of HCN channels.

  • Elements Model

    Binary Elements Kinetic Modeling

    An intuitive approach to ion channel kinetics and energetics.


Of primary interest in the Goldschen-Ohm lab is uncovering mechanisms of ion channel behavior and drug-modulation. Ion channels are critical for rapid signaling between neurons and other excitable cells necessary for both normal cognition and physiological function. They bind a variety of small molecules that modulate their activity in ways that can be used for therapies that counteract disorders of the nervous system such as epilepsy, or other cellular signaling abnormalities such as cardiac arrhythmias. By uncovering the physical mechanisms by which these molecules drive channel behavior, we will open up a new avenue for developing new therapeutic strategies that impact human health.

The lab utilizes a variety of approaches such as fluorescence spectroscopy, electrophysiology and biochemistry at both single-molecule and ensemble levels to probe the dynamic motions of proteins that underlie their biological function. Dr. Goldschen-Ohm has recently developed a new high-throughput approach that can resolve binding of a single fluorescently-tagged ligand or drug molecule to its target protein at physiologically relevant concentrations previously inaccessible to single-molecule fluorescence techniques. This advance provides a novel window through which to examine how ligand/drug binding drives molecular function at a single-molecule level. The lab is particularly interested in developing new approaches to resolve the interplay between distinct domains within individual proteins, and uncover the role of these interactions in drug binding and modulation.