@article {325,
	title = {ATP-dependent roles of the DEAD-box protein Mss116p in group II intron splicing in vitro and in vivo.},
	journal = {J Mol Biol},
	volume = {411},
	number = {3},
	year = {2011},
	month = {2011 Aug 19},
	pages = {661-79},
	abstract = {The yeast DEAD-box protein Mss116p functions as a general RNA chaperone in splicing mitochondrial group I and group II introns. For most of its functions, Mss116p is thought to use ATP-dependent RNA unwinding to facilitate RNA structural transitions, but it has been suggested to assist in the folding of one group II intron (aI5γ) primarily by stabilizing a folding intermediate. Here we compare three aI5γ constructs: one with long exons, one with short exons, and a ribozyme construct lacking exons. The long exons result in slower splicing, suggesting that they misfold and/or stabilize nonnative intronic structures. Nevertheless, Mss116p acceleration of all three constructs depends on ATP and is inhibited by mutations that compromise RNA unwinding, suggesting similar mechanisms. Results of splicing assays and a new two-stage assay that separates ribozyme folding and catalysis indicate that maximal folding of all three constructs by Mss116p requires ATP-dependent RNA unwinding. ATP-independent activation is appreciable for only a subpopulation of the minimal ribozyme construct and not for constructs containing exons. As expected for a general RNA chaperone, Mss116p can also disrupt the native ribozyme, which can refold after Mss116p removal. Finally, using yeast strains with mitochondrial DNA containing only the single intron aI5γ, we show that Mss116p mutants promote splicing in vivo to degrees that correlate with their residual ATP-dependent RNA-unwinding activities. Together, our results indicate that, although DEAD-box proteins play multiple roles in RNA folding, the physiological function of Mss116p in aI5γ splicing includes a requirement for ATP-dependent local unfolding, allowing the conversion of nonfunctional RNA structure into functional RNA structure.},
	keywords = {Adenosine Triphosphate, Blotting, Northern, DEAD-box RNA Helicases, Introns, Mitochondria, Molecular Chaperones, Polymerase Chain Reaction, RNA Helicases, RNA Splicing, RNA, Catalytic, RNA, Fungal, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins},
	issn = {1089-8638},
	doi = {10.1016/j.jmb.2011.05.047},
	author = {Potratz, Jeffrey P and Del Campo, Mark and Wolf, Rachel Z and Lambowitz, Alan M and Russell, Rick}
}
