@article {337,
	title = {DEAD-box proteins can completely separate an RNA duplex using a single ATP.},
	journal = {Proc Natl Acad Sci U S A},
	volume = {105},
	number = {51},
	year = {2008},
	month = {2008 Dec 23},
	pages = {20203-8},
	abstract = {DEAD-box proteins are ubiquitous in RNA metabolism and use ATP to mediate RNA conformational changes. These proteins have been suggested to use a fundamentally different mechanism from the related DNA and RNA helicases, generating local strand separation while remaining tethered through additional interactions with structured RNAs and RNA-protein (RNP) complexes. Here, we provide a critical test of this model by measuring the number of ATP molecules hydrolyzed by DEAD-box proteins as they separate short RNA helices characteristic of structured RNAs (6-11 bp). We show that the DEAD-box protein CYT-19 can achieve complete strand separation using a single ATP, and that 2 related proteins, Mss116p and Ded1p, display similar behavior. Under some conditions, considerably ,
	keywords = {Adenosine Triphosphate, DEAD-box RNA Helicases, Models, Chemical, Nucleic Acid Conformation, Protein Conformation, RNA, Double-Stranded, Saccharomyces cerevisiae Proteins},
	issn = {1091-6490},
	doi = {10.1073/pnas.0811075106},
	author = {Chen, Yingfeng and Potratz, Jeffrey P and Tijerina, Pilar and Del Campo, Mark and Lambowitz, Alan M and Russell, Rick}
}
