@article {338,
	title = {Toward predicting self-splicing and protein-facilitated splicing of group I introns.},
	journal = {RNA},
	volume = {14},
	number = {10},
	year = {2008},
	month = {2008 Oct},
	pages = {2013-29},
	abstract = {In the current era of massive discoveries of noncoding RNAs within genomes, being able to infer a function from a nucleotide sequence is of paramount interest. Although studies of individual group I introns have identified self-splicing and nonself-splicing examples, there is no overall understanding of the prevalence of self-splicing or the factors that determine it among the >2300 group I introns sequenced to date. Here, the self-splicing activities of 12 group I introns from various organisms were assayed under six reaction conditions that had been shown previously to promote RNA catalysis for different RNAs. Besides revealing that assessing self-splicing under only one condition can be misleading, this survey emphasizes that in vitro self-splicing efficiency is correlated with the GC content of the intron (>35\% GC was generally conductive to self-splicing), and with the ability of the introns to form particular tertiary interactions. Addition of the Neurospora crassa CYT-18 protein activated splicing of two nonself-splicing introns, but inhibited the second step of self-splicing for two others. Together, correlations between sequence, predicted structure and splicing begin to establish rules that should facilitate our ability to predict the self-splicing activity of any group I intron from its sequence.},
	keywords = {Base Composition, Base Sequence, Catalysis, Introns, Neurospora crassa, Nucleic Acid Conformation, RNA Splicing, RNA, Catalytic, RNA, Messenger, Tyrosine-tRNA Ligase},
	issn = {1469-9001},
	doi = {10.1261/rna.1027208},
	author = {Vicens, Quentin and Paukstelis, Paul J and Westhof, Eric and Lambowitz, Alan M and Cech, Thomas R}
}
