@article {346,
	title = {Do DEAD-box proteins promote group II intron splicing without unwinding RNA?},
	journal = {Mol Cell},
	volume = {28},
	number = {1},
	year = {2007},
	month = {2007 Oct 12},
	pages = {159-66},
	abstract = {The DEAD-box protein Mss116p promotes group II intron splicing in vivo and in vitro. Here we explore two hypotheses for how Mss116p promotes group II intron splicing: by using its RNA unwinding activity to act as an RNA chaperone or by stabilizing RNA folding intermediates. We show that an Mss116p mutant in helicase motif III (SAT/AAA), which was reported to stimulate splicing without unwinding RNA, retains ATP-dependent unwinding activity and promotes unfolding of a structured RNA. Its unwinding activity increases sharply with decreasing duplex length and correlates with group II intron splicing activity in quantitative assays. Additionally, we show that Mss116p can promote ATP-independent RNA unwinding, presumably via single-strand capture, also potentially contributing to DEAD-box protein RNA chaperone activity. Our findings favor the hypothesis that DEAD-box proteins function in group II intron splicing as in other processes by using their unwinding activity to act as RNA chaperones.},
	keywords = {DEAD-box RNA Helicases, Introns, Mutation, Nucleic Acid Conformation, Nucleic Acid Denaturation, Recombinant Fusion Proteins, RNA, RNA Splicing, Saccharomyces cerevisiae Proteins},
	issn = {1097-2765},
	doi = {10.1016/j.molcel.2007.07.028},
	author = {Del Campo, Mark and Tijerina, Pilar and Bhaskaran, Hari and Mohr, Sabine and Yang, Quansheng and Jankowsky, Eckhard and Russell, Rick and Lambowitz, Alan M}
}
