@article {347,
	title = {Gene targeting in gram-negative bacteria by use of a mobile group II intron ("Targetron") expressed from a broad-host-range vector.},
	journal = {Appl Environ Microbiol},
	volume = {73},
	number = {8},
	year = {2007},
	month = {2007 Apr},
	pages = {2735-43},
	abstract = {Mobile group II introns ("targetrons") can be programmed for insertion into virtually any desired DNA target with high frequency and specificity. Here, we show that targetrons expressed via an m-toluic acid-inducible promoter from a broad-host-range vector containing an RK2 minireplicon can be used for efficient gene targeting in a variety of gram-negative bacteria, including Escherichia coli, Pseudomonas aeruginosa, and Agrobacterium tumefaciens. Targetrons expressed from donor plasmids introduced by electroporation or conjugation yielded targeted disruptions at frequencies of 1 to 58\% of screened colonies in the E. coli lacZ, P. aeruginosa pqsA and pqsH, and A. tumefaciens aopB and chvI genes. The development of this broad-host-range system for targetron expression should facilitate gene targeting in many bacteria.},
	keywords = {Agrobacterium tumefaciens, Benzoates, Escherichia coli, Gene Deletion, Gene Targeting, Genetic Vectors, Gram-Negative Bacteria, Introns, Plasmids, Promoter Regions, Genetic, Pseudomonas aeruginosa},
	issn = {0099-2240},
	doi = {10.1128/AEM.02829-06},
	author = {Yao, Jun and Lambowitz, Alan M}
}
