@article {361,
	title = {The splicing of yeast mitochondrial group I and group II introns requires a DEAD-box protein with RNA chaperone function.},
	journal = {Proc Natl Acad Sci U S A},
	volume = {102},
	number = {1},
	year = {2005},
	month = {2005 Jan 4},
	pages = {163-8},
	abstract = {Group I and II introns self-splice in vitro, but require proteins for efficient splicing in vivo, to stabilize the catalytically active RNA structure. Recent studies showed that the splicing of some Neurospora crassa mitochondrial group I introns additionally requires a DEAD-box protein, CYT-19, which acts as an RNA chaperone to resolve nonnative structures formed during RNA folding. Here we show that, in Saccharomyces cerevisiae mitochondria, a related DEAD-box protein, Mss116p, is required for the efficient splicing of all group I and II introns, some RNA end-processing reactions, and translation of a subset of mRNAs, and that all these defects can be partially or completely suppressed by the expression of CYT-19. Results for the aI2 group II intron indicate that Mss116p is needed after binding the intron-encoded maturase, likely for the disruption of stable but inactive RNA structures. Our results suggest that both group I and II introns are prone to kinetic traps in RNA folding in vivo and that the splicing of both types of introns may require DEAD-box proteins that function as RNA chaperones.},
	keywords = {Amino Acid Motifs, DEAD-box RNA Helicases, Introns, Mitochondria, Molecular Chaperones, Mutation, Protein Biosynthesis, RNA, RNA Helicases, RNA Processing, Post-Transcriptional, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins},
	issn = {0027-8424},
	doi = {10.1073/pnas.0407896101},
	author = {Huang, Hon-Ren and Rowe, Claire E and Mohr, Sabine and Jiang, Yue and Lambowitz, Alan M and Perlman, Philip S}
}
