@article {389,
	title = {Characterization of an unusual tRNA-like sequence found inserted in a Neurospora retroplasmid.},
	journal = {Nucleic Acids Res},
	volume = {28},
	number = {13},
	year = {2000},
	month = {2000 Jul 1},
	pages = {1514-24},
	abstract = {We characterized an unusual tRNA-like sequence that had been found inserted in suppressive variants of the mitochondrial retroplasmid of Neurospora intermedia strain Varkud. We previously identified two forms of the tRNA-like sequence, one of 64 nt (TRL-64)and the other of 78 nt (TRL-78) containing a 14-nt internal insertion in the anticodon stem at a position expected for a nuclear tRNA intron. Here, we show that TRL-78 is encoded in Varkud mitochondrial (mt)DNA within a 7 kb sequence that is not present in Neurospora crassa wild-type 74A mtDNA. This 7-kb insertion also contains a perfectly duplicated tRNA(Trp)gene, segments of several mitochondrial plasmids and numerous GC-rich pallindromic sequences that are repeated elsewhere in the mtDNA. The mtDNA-encoded copy of TRL-78 is transcribed and apparently undergoes 5{\textquoteright}- and 3{\textquoteright}-end processing and 3{\textquoteright} nucleotide addition by tRNA nucleotidyl transferase to yield a discrete tRNA-sized molecule. However, the 14 nt intron-like sequence in TRL-78, which is missing in the TRL-64 form, is not spliced detectably in vivo or in vitro. Our results show that TRL-78 is an unusual tRNA-like species that could be incorporated into suppressive retroplasmids by the same reverse transcription mechanism used to incorporate mt tRNAs. The tRNA-like sequence may have been derived from an intron-containing nuclear tRNA gene or it may serve some function, like tmRNA. Our results suggest that mtRNAs or tRNA-like species may be integrated into mtDNA via reverse transcription, analogous to SINE elements in animal cells.},
	keywords = {Base Sequence, Blotting, Southern, Cloning, Molecular, Deoxyribonuclease EcoRI, DNA, Fungal, DNA, Mitochondrial, Genetic Variation, Introns, Mitochondria, Molecular Sequence Data, Mutagenesis, Insertional, Neurospora, Neurospora crassa, Nucleic Acid Conformation, Physical Chromosome Mapping, Plasmids, Reverse Transcriptase Polymerase Chain Reaction, RNA Processing, Post-Transcriptional, RNA, Fungal, RNA, Transfer, Trp, Transcription, Genetic},
	issn = {1362-4962},
	author = {Mohr, S and Wanner, L A and Bertrand, H and Lambowitz, A M}
}
