@article {393,
	title = {RNA and protein catalysis in group II intron splicing and mobility reactions using purified components.},
	journal = {Biochemistry},
	volume = {38},
	number = {28},
	year = {1999},
	month = {1999 Jul 13},
	pages = {9069-83},
	abstract = {Group II introns encode proteins with reverse transcriptase activity. These proteins also promote RNA splicing (maturase activity) and then, with the excised intron, form a site-specific DNA endonuclease that promotes intron mobility by reverse splicing into DNA followed by target DNA-primed reverse transcription. Here, we used an Escherichia coli expression system for the Lactococcus lactis group II intron Ll.LtrB to show that the intron-encoded protein (LtrA) alone is sufficient for maturase activity, and that RNP particles containing only the LtrA protein and excised intron RNA have site-specific DNA endonuclease and target DNA-primed reverse transcriptase activity. Detailed analysis of the splicing reaction indicates that LtrA is an intron-specific splicing factor that binds to unspliced precursor RNA with a K(d) of ,
	keywords = {Bacterial Proteins, Catalysis, Deoxyribonucleases, Type II Site-Specific, DNA Transposable Elements, Enzyme Stability, Escherichia coli, Hydrogen-Ion Concentration, Introns, Lactococcus lactis, Nucleic Acid Conformation, Recombinant Proteins, Ribonucleoproteins, RNA Splicing, RNA, Bacterial, RNA-Binding Proteins, RNA-Directed DNA Polymerase, Sodium Chloride, Substrate Specificity},
	issn = {0006-2960},
	doi = {10.1021/bi982799l},
	author = {Saldanha, R and Chen, B and Wank, H and Matsuura, M and Edwards, J and Lambowitz, A M}
}
