@article {402,
	title = {Mobility of yeast mitochondrial group II introns: engineering a new site specificity and retrohoming via full reverse splicing.},
	journal = {Cell},
	volume = {88},
	number = {6},
	year = {1997},
	month = {1997 Mar 21},
	pages = {865-74},
	abstract = {The mobile group II introns aI1 and aI2 of yeast mtDNA encode endonuclease activities that cleave intronless DNA target sites to initiate mobility by target DNA-primed reverse transcription. For aI2, sense-strand cleavage occurs mainly by a partial reverse splicing reaction, whereas for aI1, complete reverse splicing occurs, leading to insertion of the linear intron RNA into double-stranded DNA. Here, we show that aI1 homing and reverse splicing depend on the EBS1 (RNA)/IBS1(DNA) pairing and that target specificity can be changed by compensatory changes in the target site and the donor intron. Using well-marked strains to follow coconversion of flanking DNA, we show that homing occurs by both RT-dependent and -independent pathways. Remarkably, in most RT-dependent events, the reverse spliced intron is the initial template for first-strand cDNA synthesis.},
	keywords = {Base Sequence, DNA, Complementary, DNA, Fungal, DNA, Mitochondrial, Endonucleases, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Introns, Mutagenesis, Site-Directed, Plasmids, Recombination, Genetic, RNA Splicing, Yeasts},
	issn = {0092-8674},
	author = {Eskes, R and Yang, J and Lambowitz, A M and Perlman, P S}
}
