@article {4076,
	title = {Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase-Cas1 fusion protein.},
	journal = {Science},
	volume = {351},
	number = {6276},
	year = {2016},
	month = {2016 Feb 26},
	pages = {aad4234},
	abstract = {CRISPR systems mediate adaptive immunity in diverse prokaryotes. CRISPR-associated Cas1 and Cas2 proteins have been shown to enable adaptation to new threats in type I and II CRISPR systems by the acquisition of short segments of DNA (spacers) from invasive elements. In several type III CRISPR systems, Cas1 is naturally fused to a reverse transcriptase (RT). In the marine bacterium Marinomonas mediterranea (MMB-1), we showed that a RT-Cas1 fusion protein enables the acquisition of RNA spacers in vivo in a RT-dependent manner. In vitro, the MMB-1 RT-Cas1 and Cas2 proteins catalyze the ligation of RNA segments into the CRISPR array, which is followed by reverse transcription. These observations outline a host-mediated mechanism for reverse information flow from RNA to DNA.},
	issn = {1095-9203},
	doi = {10.1126/science.aad4234},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/26917774},
	author = {Silas, Sukrit and Mohr, Georg and Sidote, David J and Markham, Laura M and Sanchez-Amat, Antonio and Bhaya, Devaki and Lambowitz, Alan M and Fire, Andrew Z}
}
