@article {434,
	title = {Analysis of large deletions in the Mauriceville and Varkud mitochondrial plasmids of Neurospora.},
	journal = {Curr Genet},
	volume = {18},
	number = {4},
	year = {1990},
	month = {1990 Nov},
	pages = {365-9},
	abstract = {The Mauriceville and Varkud mitochondrial plasmids are closely related, closed-circular DNAs (3.6 and 3.7 kb, respectively) that have characteristics of mtDNA introns and retroid elements. Both plasmids contain a 710 amino acid open reading frame (ORF) that encodes an 81 kDa protein having reverse transcriptase activity. Here, we analyzed two mutant plasmids, V5-36 and M3-24, that have undergone relatively large deletions (approximately 0.35 and 0.5 kb, respectively). Both deletions occur downstream of the long ORF in a non-coding region of the plasmids that contains a direct repeat of 160 bp and a cluster of five PstI-palindromes, a repetitive sequence element in Neurospora mtDNA. In V5-36, the deletion end points are at the bases of two hairpin structures that are centered around PstI-palindromes and flank the deleted region. In M3-24, the deletion junction contains an extra T-residue that is not encoded in the plasmid. In both plasmids, the deletion end points do not correspond to homologous or directly repeated sequences of more than one nucleotide, whose pairing could account for the deletion junction. The characteristics of the deletion end points can be accounted for either by illegitimate recombination, possibly following double strand breaks at cruciform structures, or by interruption of reverse transcription followed by reinitiation downstream. The finding that the deletions encompass the 160 bp direct repeat and all five PstI-palindromes indicates that neither are required for propagation of the plasmids and supports the hypothesis that PstI-palindromes are selfish DNA elements that inserted into a nonessential region of the plasmid.},
	keywords = {Amino Acid Sequence, Base Sequence, Chromosome Deletion, DNA, Fungal, DNA, Mitochondrial, Introns, Molecular Sequence Data, Neurospora, Nucleic Acid Conformation, Open Reading Frames, Plasmids, Recombination, Genetic, Repetitive Sequences, Nucleic Acid, Restriction Mapping, Transcription, Genetic},
	issn = {0172-8083},
	author = {Akins, R A and Lambowitz, A M}
}
