@article {440,
	title = {Cloning and analysis of the Neurospora crassa gene for cytochrome c heme lyase.},
	journal = {J Biol Chem},
	volume = {264},
	number = {30},
	year = {1989},
	month = {1989 Oct 25},
	pages = {17897-906},
	abstract = {The cyt-2-1 mutant of Neurospora crassa is deficient in cytochromes aa3 and c and in cytochrome c heme lyase activity (Mitchell, M.B., Mitchell, H.K., and Tissieres, A. (1953) Proc. Natl. Acad. Sci. U.S.A. 39, 606-613; Nargang, F.E., Drygas, M.E., Kwong, P.L., Nicholson, D.W., and Neupert, W. (1988) J. Biol. Chem. 263, 9388-9394). By rescue of the slow growth character of the cyt-2-1 mutant, we have cloned the cyt-2+ gene from a N. crassa genomic library using sib selection. Analysis of the DNA sequence of the cyt-2+ gene revealed an open reading frame of 346 amino acids that has homology to the yeast cytochrome c heme lyase. The open reading frame is interrupted by two short introns. Codon usage and Northern hybridization analysis suggest that the cyt-2 gene is expressed at low levels. The cyt-2-1 mutant allele was cloned from a partial cyt-2-1 gene bank using the wild-type gene as a probe. Sequence analysis of the mutant gene revealed a 2-base (CT) deletion that alters the reading frame for 21 codons before generating an early stop codon in the protein-coding sequence. It was previously suggested that the cyt-2-1 mutation inactivates one of two regulatory circuits controlling the production of cytochrome aa3. The finding that the cyt-2-1 mutation affects the coding sequence for cytochrome c heme lyase provides a direct explanation for the deficiency of cytochrome c in the mutant and suggests that the lack of cytochrome aa3 is a regulatory response to the deficiency of cytochrome c.},
	keywords = {Amino Acid Sequence, Base Sequence, Cloning, Molecular, Escherichia coli, Genes, Fungal, Lyases, Molecular Sequence Data, Mutation, Neurospora, Neurospora crassa, Polymorphism, Restriction Fragment Length, Restriction Mapping, RNA Splicing, Saccharomyces cerevisiae, Sequence Homology, Nucleic Acid},
	issn = {0021-9258},
	author = {Drygas, M E and Lambowitz, A M and Nargang, F E}
}
