@article {441,
	title = {Mutations in nuclear gene cyt-4 of Neurospora crassa result in pleiotropic defects in processing and splicing of mitochondrial RNAs.},
	journal = {Genetics},
	volume = {123},
	number = {1},
	year = {1989},
	month = {1989 Sep},
	pages = {97-108},
	abstract = {The nuclear cyt-4 mutants of Neurospora crassa have been shown previously to be defective in splicing the group I intron in the mitochondrial large rRNA gene and in 3{\textquoteright} end synthesis of the mitochondrial large rRNA. Here, Northern hybridization experiments show that the cyt-4-1 mutant has alterations in a number of mitochondrial RNA processing pathways, including those for cob, coI, coII and ATPase 6 mRNAs, as well as mitochondrial tRNAs. Defects in these pathways include inhibition of 5{\textquoteright} and 3{\textquoteright} end processing, accumulation of aberrant RNA species, and inhibition of splicing of both group I introns in the cob gene. The various defects in mitochondrial RNA synthesis in the cyt-4-1 mutant cannot be accounted for by deficiency of mitochondrial protein synthesis or energy metabolism, and they suggest that the cyt-4-1 mutant is defective in a component or components required for processing and/or turnover of a number of different mitochondrial RNAs. Defective splicing of the mitochondrial large rRNA intron in the cyt-4-1 mutant may be a secondary effect of failure to synthesize pre-rRNAs having the correct 3{\textquoteright} end. However, a similar explanation cannot be invoked to account for defective splicing of the cob pre-mRNA introns, and the cyt-4-1 mutation may directly affect splicing of these introns.},
	keywords = {Blotting, Northern, Electrophoresis, Polyacrylamide Gel, Introns, Mitochondria, Mutation, Neurospora, Neurospora crassa, Polymorphism, Restriction Fragment Length, Restriction Mapping, RNA, RNA Probes, RNA Splicing, Transcription, Genetic},
	issn = {0016-6731},
	author = {Dobinson, K F and Henderson, M and Kelley, R L and Collins, R A and Lambowitz, A M}
}
