@article {450,
	title = {A 3{\textquoteright} splice site mutation in a nuclear gene encoding a mitochondrial ribosomal protein in Neurospora crassa.},
	journal = {J Biol Chem},
	volume = {263},
	number = {6},
	year = {1988},
	month = {1988 Feb 25},
	pages = {2848-52},
	abstract = {We showed previously that the cyt-21+ gene of Neurospora crassa encodes a mitochondrial ribosomal protein homologous to Escherichia coli ribosomal protein S-16 (Kuiper, M. T. R., Akins, R. A., Holtrop, M., de Vries, H., and Lambowitz, A. M. (1988) J. Biol. Chem. 263, 2840-2847). A mutation in this gene, cyt-21-1, results in deficiency of mitochondrial small ribosomal subunits and small rRNA (Collins, R. A., Bertrand, H., LaPolla, R. J., and Lambowitz, A. M. (1979) Mol. Gen. Genet. 177, 73-84). In the present work, cloning and sequencing of the cyt-21-1 mutant allele show that it contains a single dG to dA transition at the 3{\textquoteright} splice site AG of the first intron in the protein coding region. This mutation leads to inactivation of the normal 3{\textquoteright} splice site and activation of a cryptic 3{\textquoteright} splice site, 15 nucleotides downstream. The use of this cryptic splice site results in an in-frame deletion of 5 amino acids from the cyt-21 protein. Comparison of mutant and wild-type mitochondrial small ribosomal subunit proteins showed one protein, S-24, with an altered electrophoretic mobility, consistent with the predicted deletion. The mutant ribosomal protein is still capable of binding to mitochondrial small ribosomal subunits, but results in abnormal mitochondrial ribosome assembly.},
	keywords = {Alleles, Amino Acid Sequence, Base Sequence, Endonucleases, Genes, Fungal, Mutation, Neurospora, Neurospora crassa, Ribosomal Proteins, RNA, Fungal, Single-Strand Specific DNA and RNA Endonucleases},
	issn = {0021-9258},
	author = {Kuiper, M T and Holtrop, M and Vennema, H and Lambowitz, A M and de Vries, H}
}
