@article {451,
	title = {Isolation and analysis of the Neurospora crassa Cyt-21 gene. A nuclear gene encoding a mitochondrial ribosomal protein.},
	journal = {J Biol Chem},
	volume = {263},
	number = {6},
	year = {1988},
	month = {1988 Feb 25},
	pages = {2840-7},
	abstract = {The Neurospora crassa nuclear mutant cyt-21-1 (originally 297-24; Pittenger, T.H., and West, D.J. (1979) Genetics 93, 539-555) has a defect leading to gross deficiency of mitochondrial small ribosomal subunits. Here, we have cloned the cyt-21+ gene from a N. crassa genomic library, using the sib selection procedure (Akins, R. A., and Lambowitz, A. M. (1985) Mol. Cell Biol. 5, 2272-2278). The genomic clone contains a short split gene encoding a basic protein of 107 amino acid residues. This protein shows strong homology to Escherichia coli ribosomal protein S-16. Comparison of mutant and wild-type mitochondrial ribosomal proteins (Kuiper, M. T. R., Holtrop, M., Vennema, H., Lambowitz, A. M., and de Vries, H. (1988) J. Biol. Chem. 263, 2848-2852) indicates that the cyt-21 gene encodes N. crassa mitochondrial ribosomal protein S-24. The expression of the cyt-21+ gene is regulated such that the level of the putative cyt-21+ mRNA is increased about 5-fold when mitochondrial protein synthesis is inhibited. We suggest that this reflects part of a general mechanism for coordinately activating Neurospora nuclear genes that encode mitochondrial constituents in response to impaired mitochondrial function. This is the first report of the cloning and characterization of a mitochondrial ribosomal protein gene from N. crassa.},
	keywords = {Amino Acid Sequence, Base Sequence, DNA Restriction Enzymes, Genes, Fungal, Mitochondria, Molecular Sequence Data, Mutation, Neurospora, Neurospora crassa, Nucleic Acid Hybridization, Ribosomal Proteins, Transcription, Genetic},
	issn = {0021-9258},
	author = {Kuiper, M T and Akins, R A and Holtrop, M and de Vries, H and Lambowitz, A M}
}
