@article {453,
	title = {Molecular cloning and characterization of the cys-3 regulatory gene of Neurospora crassa.},
	journal = {Mol Cell Biol},
	volume = {7},
	number = {7},
	year = {1987},
	month = {1987 Jul},
	pages = {2506-11},
	abstract = {The regulatory gene cys-3+ controls the synthesis of a number of enzymes involved in sulfur metabolism. cys-3 mutants show a multiple loss of enzymes in different pathways of sulfur metabolism. The cys-3+ gene was isolated by transformation of an aro-9 qa-2 cys-3 inl strain with a clone bank followed by screening with the "sib selection" method. The library used (pRAL1) contained inserts of Sau3a partial digest fragments of about 9 kilobases as well as the Neurospora qa-2+ gene. Double selection for qa-2+ and cys-3+ function was carried out. The transformants obtained with the isolated cys-3+ clone show recovery of the enzyme activities associated with the cys-3 mutation (e.g., arylsulfatase and sulfate permease). Restriction fragment length polymorphism experiments confirmed the identity of the clone, mRNA studies with Northern blots show that the expression of the cys-3+ gene is inducible. In contrast to cys-3+, the cys-3 (P22) mutant gene was not expressed at a higher level under sulfur-derepressed conditions.},
	keywords = {Cloning, Molecular, Gene Expression Regulation, Genes, Fungal, Genes, Regulator, Neurospora, Neurospora crassa, Polymorphism, Restriction Fragment Length, Sulfur, Transformation, Genetic},
	issn = {0270-7306},
	author = {Paietta, J V and Akins, R A and Lambowitz, A M and Marzluf, G A}
}
