@article {457,
	title = {Mechanism of recognition of the 5{\textquoteright} splice site in self-splicing group I introns.},
	journal = {Nature},
	volume = {322},
	number = {6074},
	year = {1986},
	month = {1986 Jul 3-9},
	pages = {86-9},
	abstract = {Group I introns include many mitochondrial ribosomal RNA and messenger RNA introns and the nuclear rRNA introns of Tetrahymena and Physarum. The splicing of precursor RNAs containing these introns is a two-step reaction. Cleavage at the 5{\textquoteright} splice site precedes cleavage at the 3{\textquoteright} splice site, the latter cleavage being coupled with exon ligation. Following the first cleavage, the 5{\textquoteright} exon must somehow be held in place for ligation. We have now tested the reactivity of two self-splicing group I RNAs, the Tetrahymena pre-rRNA and the intron 1 portion of the Neurospora mitochondrial cytochrome b (cob) pre-mRNA, in the intermolecular exon ligation reaction (splicing in trans) described by Inoue et al. The different sequence specificity of the reactions supports the idea that the nucleotides immediately upstream from the 5{\textquoteright} splice site are base-paired to an internal, 5{\textquoteright} exon-binding site, in agreement with RNA structure models proposed by Davies and co-workers and others. The internal binding site is proposed to be involved in the formation of a structure that specifies the 5{\textquoteright} splice site and, following the first step of splicing, to hold the 5{\textquoteright} exon in place for exon ligation.},
	keywords = {Animals, Base Sequence, Cytochrome b Group, Neurospora, Nucleic Acid Conformation, Nucleic Acid Precursors, Physarum, RNA Precursors, RNA Splicing, RNA, Messenger, RNA, Ribosomal, Tetrahymena},
	issn = {0028-0836},
	doi = {10.1038/322086a0},
	author = {Garriga, G and Lambowitz, A M and Inoue, T and Cech, T R}
}
