@article {480,
	title = {Mitochondrial ribosome assembly in Neurospora crassa. Purification of the mitochondrially synthesized ribosomal protein, S-5.},
	journal = {J Biol Chem},
	volume = {256},
	number = {13},
	year = {1981},
	month = {1981 Jul 10},
	pages = {7064-7},
	abstract = {In Neurospora, one mitochondrial ribosomal protein (S-5, Mr = 52,000) is synthesized intramitochondrially and is presumably encoded by mitochondrial DNA. We have developed a rapid method for the purification of S-5 which takes advantage of its high affinity for carboxymethyl-Sepharose in the presence of 6 M urea. Using this method, S-5, at purity greater than 95\%, can be prepared by column chromatography in a single batch elution step. The amino acid composition of S-5 was determined. Judged by the contents of hydrophilic and basic amino acids, S-5 is more similar to Escherichia coli and yeast ribosomal proteins than to other mitochondrial translation products which are hydrophobic membrane proteins.},
	keywords = {Amino Acids, DNA, Mitochondrial, Macromolecular Substances, Mitochondria, Molecular Weight, Neurospora, Neurospora crassa, Ribosomal Proteins, Ribosomes},
	issn = {0021-9258},
	author = {Lapolla, R J and Lambowitz, A M}
}
