@article {5931,
	title = {Efficient and quantitative high-throughput tRNA sequencing.},
	journal = {Nat Methods},
	volume = {12},
	number = {9},
	year = {2015},
	month = {2015 Sep},
	pages = {835-7},
	abstract = {Despite its biological importance, tRNA has not been adequately sequenced by standard methods because of its abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieved efficient and quantitative tRNA sequencing in HEK293T cells by using engineered demethylases to remove base methylations and a highly processive thermostable group II intron reverse transcriptase to overcome these obstacles. Our method, DM-tRNA-seq, should be applicable to investigations of tRNA in all organisms.},
	keywords = {Algorithms, Base Sequence, Gene Library, HEK293 Cells, High-Throughput Nucleotide Sequencing, Humans, Molecular Sequence Data, RNA, Transfer},
	issn = {1548-7105},
	doi = {10.1038/nmeth.3478},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/26214130},
	author = {Zheng, Guanqun and Qin, Yidan and Clark, Wesley C and Dai, Qing and Yi, Chengqi and He, Chuan and Lambowitz, Alan M and Pan, Tao}
}
