@article {9224,
	title = {Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching.},
	journal = {Scientific Reports},
	volume = {7},
	number = {8421},
	year = {2017},
	abstract = {High-throughput single-stranded DNA sequencing (ssDNA-seq) of cell-free DNA from plasma and other bodily fluids is a powerful method for non-invasive prenatal testing, and diagnosis of cancers and other diseases. Here, we developed a facile ssDNA-seq method, which exploits a novel template-switching activity of thermostable group II intron reverse transcriptases (TGIRTs) for DNA-seq library construction. This activity enables TGIRT enzymes to initiate DNA synthesis directly at the 3' end of a DNA strand while simultaneously attaching a DNA-seq adapter without end repair, tailing, or ligation. Initial experiments using this method to sequence\&nbsp;E. coli\&nbsp;genomic DNA showed that the TGIRT enzyme has surprisingly robust DNA polymerase activity. Further experiments showed that TGIRT-seq of plasma DNA from a healthy individual enables analysis of nucleosome positioning, transcription factor-binding sites, DNA methylation sites, and tissues-of-origin comparably to established methods, but with a simpler workflow that captures precise DNA ends.},
	url = {https://www.nature.com/articles/s41598-017-09064-w},
	author = {Douglas C. Wu and Alan M. Lambowitz}
}
