%0 Journal Article %J Nat Methods %D 2015 %T Efficient and quantitative high-throughput tRNA sequencing. %A Zheng, Guanqun %A Qin, Yidan %A Clark, Wesley C %A Dai, Qing %A Yi, Chengqi %A He, Chuan %A Lambowitz, Alan M %A Pan, Tao %K Algorithms %K Base Sequence %K Gene Library %K HEK293 Cells %K High-Throughput Nucleotide Sequencing %K Humans %K Molecular Sequence Data %K RNA, Transfer %X Despite its biological importance, tRNA has not been adequately sequenced by standard methods because of its abundant post-transcriptional modifications and stable structure, which interfere with cDNA synthesis. We achieved efficient and quantitative tRNA sequencing in HEK293T cells by using engineered demethylases to remove base methylations and a highly processive thermostable group II intron reverse transcriptase to overcome these obstacles. Our method, DM-tRNA-seq, should be applicable to investigations of tRNA in all organisms. %B Nat Methods %V 12 %P 835-7 %8 2015 Sep %G eng %U https://www.ncbi.nlm.nih.gov/pubmed/26214130 %N 9 %1 http://www.ncbi.nlm.nih.gov/pubmed/26214130?dopt=Abstract %R 10.1038/nmeth.3478