Primer Sequences

N-terminal deletion construction

Primer Name

Description

Sequence (5’ à 3’)

 

Bush F1

5’ end, BamHI site

acattttgctgccggtcaGGATCCATGAGGATTGAGAGGCTTC

 

Bush R1

 

3’ end, XhoI site

AGTGTGTCCTTTGTCGATACTGCTCGAGTTAAGCATTTACATTGCCTC

 

Bush F5

Forward primer for 3’ fragment (with R1)

TGCAGGTCGAGGCATTCAACT

Bush R5

Reverse primer for 5’ fragment (with F1)

GACCTTCAGGAATCTTCCAC

Bush R3

For PCR colony screening (with F5)

CATCGAACCACACGAACTAC

 

Bacterially expressed antigen construct

Primer Name

Description

Sequence (5’ à 3’)

ACA1Ag_F#2

Forward primer

GGCATGGGTAGGTGGTGGTAT

ACA1Ag_R

Reverse primer

TACTGCCACTCCTGCCTTCT

 

 

LUX™ primers for Real Time RT-PCR

Primer Name

Description

Sequence (5’ à 3’)

Cr-ACA1ORF_2364FL

CrACA1 labeled forward

GACTGCTTAGGTGAGGTGGTGGCAG[FAM]C

Cr-ACA1ORF_2364FL_2413RU

CrACA1 unlabeled reverse

TCGATGCCCATTGCTAGACC

Cr-Actin_974FL

Control gene labeled forward

CTACTTGTCACCGCTCTTGCTCCAAG[JOE]AG

Cr-Actin_974FL_1055RU

Control gene unlabeled reverse

tgcaatccacatctgttgaaagg

Cr-alphaTubulin_1224FL

Control gene labeled forward

Cactttactggtggtgatctggctaaag[joe]g

Cr-alphaTubulin_1224FL_1252RU

Control gene unlabeled reverse

ACACTCTGCAACACTTGTGGA

Cr-APT_460FL

Control gene labeled forward

CACGGGTGCAAGAGGGTGACCG[JOE]G

Cr-APT_460FL_525RU

Control gene unlabeled reverse

CCACCTCTGCTCCAGCTCTC

Cr-UbiORF_392FL

Control gene labeled forward

GTACGCAAACAGTTGGAGGATGGGCG[JOE]AC

Cr-UbiORF_392FL_429RU

Control gene unlabeled reverse

GCAGACGCAGCACCAAATGA