N-terminal deletion construction |
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Primer Name |
Description |
Sequence (5’ à 3’) |
|
Bush F1 |
5’ end, BamHI site |
acattttgctgccggtcaGGATCCATGAGGATTGAGAGGCTTC |
|
Bush R1 |
3’ end, XhoI site |
AGTGTGTCCTTTGTCGATACTGCTCGAGTTAAGCATTTACATTGCCTC |
|
Bush F5 |
Forward primer for 3’ fragment (with R1) |
TGCAGGTCGAGGCATTCAACT |
|
Bush R5 |
Reverse primer for 5’ fragment (with F1) |
GACCTTCAGGAATCTTCCAC |
|
Bush R3 |
For PCR colony screening (with F5) |
CATCGAACCACACGAACTAC |
Bacterially expressed antigen construct |
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Primer Name |
Description |
Sequence (5’ à 3’) |
ACA1Ag_F#2 |
Forward primer |
GGCATGGGTAGGTGGTGGTAT |
ACA1Ag_R |
Reverse primer |
TACTGCCACTCCTGCCTTCT |
LUX™ primers for Real Time RT-PCR |
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Primer Name |
Description |
Sequence (5’ à 3’) |
Cr-ACA1ORF_2364FL |
CrACA1 labeled forward |
GACTGCTTAGGTGAGGTGGTGGCAG[FAM]C |
Cr-ACA1ORF_2364FL_2413RU |
CrACA1 unlabeled reverse |
TCGATGCCCATTGCTAGACC |
Cr-Actin_974FL |
Control gene labeled forward |
CTACTTGTCACCGCTCTTGCTCCAAG[JOE]AG |
Cr-Actin_974FL_1055RU |
Control gene unlabeled reverse |
tgcaatccacatctgttgaaagg |
Cr-alphaTubulin_1224FL |
Control gene labeled forward |
Cactttactggtggtgatctggctaaag[joe]g |
Cr-alphaTubulin_1224FL_1252RU |
Control gene unlabeled reverse |
ACACTCTGCAACACTTGTGGA |
Cr-APT_460FL |
Control gene labeled forward |
CACGGGTGCAAGAGGGTGACCG[JOE]G |
Cr-APT_460FL_525RU |
Control gene unlabeled reverse |
CCACCTCTGCTCCAGCTCTC |
Cr-UbiORF_392FL |
Control gene labeled forward |
GTACGCAAACAGTTGGAGGATGGGCG[JOE]AC |
Cr-UbiORF_392FL_429RU |
Control gene unlabeled reverse |
GCAGACGCAGCACCAAATGA |