Frozen Yeast Stocks

  • mating type is indicated by a or al (alpha) 
  • $ = delta (deletion or disruption) 
  • Clicking on the linked text will take you to the reference for that strain
  • Location is listed as column\row, except for Sherwin Chan's boxes (SC1, SC2) which use row # column letter

 

Strain Genotype Phenotype Source/Ref Box Col\Row
DBY1826 a ade2 his3-$200 leu2-3,112 ura3-52 good sporulator C. Chan 1 1,2\1
DBY1827 al his3-$200 leu2-3,112 ura3-52 good sporulator C. Chan 1 3,4\1
DBY1828 a ade2 his3-$200 leu2-3,112 trp1-1 ura3-52 good sporulator C. Chan 1 5,6\1
DBY1829 al his3-$200 leu2-3,112 lys2-801 trp1-1 ura3-52 good sporulator C. Chan 1 7,8\1
2180 SUC2 mal mel gal2 CUP1 wildtype (WT) YGSC 1 9\1,2
DAY1 a ser1 leu1 tup rho- dTMP-perm; petite DRA 1 1,2\2
DAY4 a ser1 ura3-52 trp1 leu2 his4 ADE3+ DRA 1 3,4\2
3-5281 al ser1 ura1-1 ade3-130 his- ade3 deletion; his- E. Jones 1 5,6\2
DAY3 a ser1 ura3-52 trp1 leu2 ade3-130 (his-) ade3 deletion; his- DRA 1 7\2,3
M1614C a ser1 leu1 ADE3+ E. Jones 1 1,2\3
DAY2 al ser1 leu2 tup rho+ dTMP-perm; grande DRA 1 3,4\3
JRY476 al his3 his4 trp1 leu2 ura3 Sir4ts   J. Rine 1 5,6\3
1001 al ade2-1 trp1-1 leu2-3,112 his3-11,15 ura3-1 WT @ FMT B.Purnelle 1 1\4
1002 a ade2-1 trp1-1 leu2-3,112 his3-11,15 ura3-1 WT @ FMT B.Purnelle 1 2\4
1049 al ade2-1 trp1-1 leu2-3,112 his3-11,15 ura3-1 fmt::URA3 disrupted @ FMT B.Purnelle 1 3\4
1049 al ade2-1 trp1-1 leu2-3,112 his3-11,15 ura3-1 fmt::URA3 disrupted @ FMT B.Purnelle 6 7\5,6
1052 a ade2-1 trp1-1 leu2-3,112 his3-11,15 ura3-1 fmt::URA3 disrupted @ FMT B.Purnelle 1 4\4
1054 diploid of 1001 X 1002 FMT/fmt::URA3 heterozygote? B.Purnelle 1 5\4
TR2 a trp1-289 his3-600 ura3-52 lys2-801a ade2-101 mating type tester C. Guthrie 1 6\4,5
TR3 al trp1-289 his3-600 ura3-52 lys2-801a ade2-101 mating type tester C. Guthrie 1 7\4,5
X1056-1C al ade8 his3 met2 leu1 lys7 trp1 gal7 ade- YGSC 1 1\5
STX320-3D a ade8 his3 met2 leu1 lys7 gal7? ade- YGSC 1 2\5
CBY3 a ser1 ura3-52 trp1 leu2 his4 ade3-144/257 S+D-C- leaky ade+ C.Barlowe 1 1\6,7
CBY5 a ser1 ura3-52 trp1 leu2 his4 ade3-30/65 S+D-C-leaky ade+ C.Barlowe 1 2\6,7
CBY1 a ser1 ura3-52 trp1 leu2 his4 ade3-30 S-D+C+ ade+ C.Barlowe 1 3\6,7
CBY2 a ser1 ura3-52 trp1 leu2 his4 ade3-144 S+D-C- leaky ade+ C.Barlowe 1 4\6,7
CBY4 a ser1 ura3-52 trp1 leu2 his4 ade3-65 S+D-C+ ade+ C.Barlowe 1 5\6,7
CBY6 a ser1 ura3-52 trp1 leu2 his4 ade3-30/65/144 S-D-C- ade+ C.Barlowe 1 6\6,7
KSY1 al ade3-130 ura3-52 ser1 MIS1 ade- K.Shannon 1 7\6
KSY6 a ade3-130 ade2 ura3-52 ser1 mis1::URA3 ade- K.Shannon 1 8\6
KSY7 al ade3-130 ura3-52 ser1 MIS1 ade- K.Shannon 1 9\6
KSY2 a ade3-130 ura3-52 ser1 MIS1 ade- K.Shannon 1 7\7
KSY9 a ura3-52 ser1 mis1::URA3 ade+ K.Shannon 1 8\7
KSY8 al ade3-130 ura3-52 ser1 mis1::URA3 ade- K.Shannon 1 9\7
Y536 al ura3-52 lys2$201 leu2-3,112 his3$200 bas1::HIS3   Bertrand 1 1\8,9
Y549 al ura3-52 lys2$201 leu2-3,112 his3$200 BAS1   Bertrand 1 2\8,9
DKB28       1 3\8
DKB148       1 4\8
DKB147       1 3\9
UTL-7A a ura3-52 leu2-3,112 trp1 (pBSD-2) DHFR1 gene B. Barclay 1 4\9
g610-5d a fol2-1 (tmp2-10)ade3 mal0 ura1? require folinic acid J. Game 1 5\8
BR414-4A al fol2-1 (tmp2-10) require folinic acid J. Game 1 6\8
g615-1D a fol1-6 (tmp2-6) trp1 require folinic acid J. Game 1 5\9
g615-4B al fol2-1 (tmp2-10) TRP1 require folinic acid J. Game 1 6\9
M1614C tup  a ser1 leu1 tup thymidylate uptake DRA

 

 

9,10\3 
MWY4.1 a ser1 ura3-52 trp1 leu2 his4 ade3-30 $mtd1 S-N- (ade+) M. West 2 1\1,2
MWY4.4 a ser1 ura3-52 trp1 leu2 his4 ade3-65 $mtd1 D-N- (ade+) M. West 2 2\1,2,3
MWY4 a ser1 ura3-52 trp1 leu2 his4 $mtd1 N- (ade+) M. West 2 3\1,2
MWY4.5 a ser1 ura3-52 trp1 leu2 his4 ade3-30/65 $mtd1 S-D-N- (ade-) M. West 2 4\1,2
MWY4.6 a ser1 ura3-52 trp1 leu2 his4 ade3-30/65/144 $mtd1 S-D-N-C- (ade-) M. West 2 5\1,2
MWY3 a ser1 ura3-52 trp1 leu2 ade3-130 (his-) $mtd1 N- (ade- his-) M. West 2 6\1,2
MWY5 HIS4+ version of DAY4 ade+ his+ M. West 2 7\1
MWY6 HIS4+ version of MWY4 ade+ his+ M. West 2 8\1
MWY7 HIS4+ version of CBY1 ade+ his+ M. West 2 9\1
MWY8 HIS4+ version of MWY4.4 ade+ his+ M. West 2 7\2
MWY9 HIS4+ version of CBY6 ade+ his+ M. West 2 8\2
CBY6$mis1 a ser1 ura3-52 trp1 leu2 his4 ade3-30/65/144 $mis1 ura- Smitha K. 2 1,2\3
CBY4$mis1/pSyn a ser1 ura3-52 trp1 leu2 his4 ade3-65 $mis1 pSyn   M. West 2 4\3
DAY3$mis1/pSyn a ser1 ura3-52 trp1 leu2 ade3-130 $mis1 (his-) pSyn   M. West 2 5\3
DAY4$mis1/pSyn a ser1 ura3-52 trp1 leu2 his4 $mis1 pSyn   M. West 2 6\3
DAY4/YEp13-3 a ser1 ura3-52 trp1 leu2 his4 YEp13-3 MTD1 plasmid M. West 2 7,8\3
CBY6mtd1::URA3 CBY6 with mtd1::URA3 + YEp13-3 MTD1 plasmid M. West 2 9\3
MYW3/pSin-23 MWY3 with inactive synthetase domain   M. West 2 1\4
MWY3/pSin-30 MWY3 with inactive synthetase domain   M. West 2 2\4
MWY3/pS MWY3 with active synthetase domain   M. West 2 3\4
DAY3/pSin-23 DAY3 with inactive synthetase domain   M. West 2 4\4
DAY3/pSin-30 DAY3 with inactive synthetase domain   M. West 2 5\4
DAY3/pS DAY3 with active synthetase domain   M. West 2 6\4
MWY3/pADE3 MWY3 with full-length ADE3   M. West 2 7\4
DAY3/pADE3 DAY3 with full-length ADE3   M. West 2 8\4
DAY3/pVT103U DAY3 with vector   M. West 2 9\4
DAY3/pVT101U DAY3 with vector   M. West 2 9\5
MWY3/pDCin MWY3 with inactive DC domain   M. West 2 1\5
DAY3/pDCin DAY3 with inactive DC domain   M. West 2 2\5
MWY3/pDC MWY3 with active DC domain   M. West 2 3\5
DAY3/pDC DAY3 with active DC domain   M. West 2 4\5
MWY3/pVT101U MWY3 with vector   M. West 2 8\5
DAY4$mis1 pHM53 DAY4$mis1 (ura-) still contains pHM53 (Leu+)   M. West 2 1,2\6,7
DAY3$mis1 pHM53 DAY3$mis1 (ura-) still contains pHM53 (Leu+)   M. West 2 3,4\6
MWY4.1mis1::URA3 a ser1 ura3-52 trp1 leu2 his4 ade3-30 $mtd1 mis1::URA3 S-N- (ade+ ura+) M. West 2 5,6\6
MWY4.1mis1::URA3 same as MWY4.1mis1::URA3 but contains pHM53 (ade+ ura+ leu+) M. West 2 7,8\6
CBY4$mis1 pHM53 a ser1 ura3-52 trp1 leu2 his4 ade3-65 $mis1 (+ pHM53) D- (ade+ ura- leu+) M. West 2 9\6
MWY4.4$mis1pHM53 a ser1 ura3-52 trp1 leu2 his4 ade3-65 $mtd1 $mis1 (pHM53) D-N-(ade+ura-leu+) M. West 2 3,4\7
MWY4mis1::URA3 a ser1 ura3-52 trp1 leu2 his4 $mtd1 mis1::URA3 N-(ade+ura+leu-) M. West 2 5,6\7
CBY4mis1::URA3 a ser1 ura3-52 trp1 leu2 his4 ade3-65 mis1::URA3 D- (ade+ ura+ leu-) M. West 2 7\7
CBY6mis1::URA3 a ser1 ura3-52 trp1 leu2 his4 ade3-30/65/144 mis1::URA3 ade+ura+leu- M. West 2 8\7
CBY6$mis1 a ser1 ura3-52 trp1 leu2 his4 ade3-30/65/144 $mis1 ade+ura-leu- M. West 2 9\7,8
F33 al met7 gal2 pet   YGSC 2 1\8
S1896D a met7 trp1 leu1 ade1 gal1 gal2 pet   YGSC 2 2\8
JLY1053 a his3-11,15leu2-3,112trp1-1ura3-1can1-100ade2-1 aac2::HIS3 aac1::LEU2 J. Lawson 2 3\8
17-14 a his1 mating type tester C. Chan 2 4,5\8
17-17 al his1 mating type tester C. Chan 2 6,7\8
X5918-76       2 8\8
1402 al ura3-52 lys2-801am   C. Chan 4 2,3,4\3
Y5 a lpd1 leu2-3,13 met5 met2 gal2 canR petite D. Sinclair 2 2\9
Y6 al lpd1 leu2-3,13 met5 met2 gal2 canR petite D. Sinclair 2 3\9
DC-SM-1 DC saturation mutants in KSY7 ade- G/F- C.Barlowe 3 1\1
DC-SM-2 DC saturation mutants in KSY7 ade- G/F- C.Barlowe 3 2\1
DC-SM-3 DC saturation mutants in KSY7 ade- G/F- C.Barlowe 3 3\1
DC-SM-17 DC saturation mutants in KSY7 ade- G/F- C.Barlowe 3 4\1
DC-SM-18 DC saturation mutants in KSY7 ade- G/F- C.Barlowe 3 5\1
DC-SM-27 DC saturation mutants in KSY7 ade- G/F- C.Barlowe 3 6\1
DC-SM-28 DC saturation mutants in KSY7 ade- G/F- C.Barlowe 3 7\1
DC-SM-38 DC saturation mutants in KSY7 ade- G/F- C.Barlowe 3 8\1
KSY7/pU2Syn$S/X     C.Barlowe 3 9\1
KSY7/oligo4 SDM(A)     C.Barlowe 3 1\2
KSY7/oligo4 SDM(B)     C.Barlowe 3 2\2
23B synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 3\2
31A synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 4\2
7D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 5\2
1D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 6\2
13C synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 7\2
2D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 8\2
13D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 9\2
41A synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 1\3
12C synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 2\3
10C synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 3\3
23A synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 4\3
40A synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 5\3
11D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 6\3
30A synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 7\3
5D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 8\3
10A synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 9\3
8D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 1\4
3D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 2\4
29A synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 3\4
9D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 4\4
6D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 5\4
12D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 6\4
4D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 7\4
42A synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 8\4
9A synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 9\4
24A synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 1\5
10D synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 2\5
3B synthetase domain saturation mutants in DAY3 ade- G/F- C.Barlowe 3 3\5
DAY3 ser11     C.Barlowe 3 1\6
DAY3 ser144     C.Barlowe 3 2\6
DAY3 pU3$Cla     C.Barlowe 3 3\6
DAY3 pU2$D/C     C.Barlowe 3 4\6
DAY3 pU3$D/C     C.Barlowe 3 5\6
DAY3 ser257     C.Barlowe 3 6\6
DAY3-144/257     C.Barlowe 3 7\6
DAY3 pU1-CaS     C.Barlowe 3 8\6
DAY3 pU2-Syn     C.Barlowe 3 9\6
DAY3 pRS$Syn     C.Barlowe 3 1\7
DAY3 syn65     C.Barlowe 3 2\7
DAY3 syn69     C.Barlowe 3 3\7
KSY7-69     C.Barlowe 3 4\7
KSY8-54     C.Barlowe 3 5\7
KSY7-12     C.Barlowe 3 6\7
KSY7-C     C.Barlowe 3 7\7
KSY8-30     C.Barlowe 3 8\7
KSY7-30/65     C.Barlowe 3 9\7
KSY7-54     C.Barlowe 3 1\8
KSY7-G     C.Barlowe 3 2\8
KSY7-30     C.Barlowe 3 3\8
KSY8-69     C.Barlowe 3 4\8
KSY8-65     C.Barlowe 3 5\8
KSY7-30/65     C.Barlowe 3 6\8
KSY7-11     C.Barlowe 3 7\8
KSY7-65     C.Barlowe 3 8\8
KSY7-72     C.Barlowe 3 9\8
KSY8-30/65/144     C.Barlowe 3 1,2\9
KSY8 pU3.3Syn     C.Barlowe 3 3\9
DAY4 YIp65     C.Barlowe 3 4\9
CBY1 YIp8     C.Barlowe 3 5\9
PTY43 a psd2::HIS3 ura3 leu2 his3 trp1 ade2 suc2 add ethanolamine P. Trotter 4 1\1,2
PTY44 al psd1::TRP1 psd2::TRP1 ura3 leu2 his3 trp1 lys2 add ethanolamine P. Trotter 4 2\1,2
PTY44 al psd1::TRP1 psd2::TRP1 ura3 leu2 his3 trp1 lys2 add ethanolamine P. Trotter 6 8\1,2
PTY11 a psd::TRP1 trp1 ade1 gal1 gal2 add ethanolamine P. Trotter 4 3\1,2
PTY29       6 9\1,2
PTY33       6 10\1,2
EKY1 a ser1 ura3-52 trp1 leu2 his4 shm1::URA3 SHM2 mito SHMT mutant E. Kassini 4 5\1,2
EKY2 a ser1 ura3-52 trp1 leu2 his4 SHM1 shm2::LEU2 cyto SHMT mutant E. Kassini 4 6\1,2
EKY3 (EKY1-2) a ser1 ura3-52 trp1 leu2 his4 shm1::URA3 shm2::LEU2 double SHMT mutant E. Kassini 4 4\1,2
Trp1a a trp1 ade1 gal1 gal2 parent of PTY strains P. Trotter 4 1\3,4
DAY4 a/al a/al ser1 ura3-52 trp1 leu2 his4 diploid R.Raymond 4 2-5\4
DAY4 a ser1 ura3-52 trp1 leu2 his4 haploid DRA 4 1,2\5
DAY3/pAAB250 DAY3 with HO endonuclease plasmid for making diploid   R.Raymond 4 3\5
RRY1 a a ser1 ura3-52 trp1 leu2 his4 $met13 MET12   R.Raymond 4 1\6,7
RRY1 al al ser1 ura3-52 trp1 leu2 his4 $met13 MET12   R.Raymond 4 2\6,7
RRY1 a/al a/al ser1 ura3-52 trp1 leu2 his4 $met13 MET12 diploid R.Raymond 4 3\6,7
RRY2 a a ser1 ura3-52 trp1 leu2 his4 $met12 MET13   R.Raymond 4 4\6,7
RRY2 al al ser1 ura3-52 trp1 leu2 his4 $met12 MET13   R.Raymond 4 5\6,7
RRY2 a/al a/al ser1 ura3-52 trp1 leu2 his4 $met12 MET13 diploid R.Raymond 4 6\6,7
RRY3 a a ser1 ura3-52 trp1 leu2 his4 $met12 $met13 met- R.Raymond 4 7\6,7
RRY3 al al ser1 ura3-52 trp1 leu2 his4 $met12 $met13 met- R.Raymond 4 8\6,7
SDYa a ser1 ura3-52 trp1 leu2 his4 met6::URA3 met- S. Dabrowski

 

 4 

 

 9\5 
SDYal al ser1 ura3-52 trp1 leu2 his4 met6::URA3 met-  S. Dabrowski

 

 4 

 

 10\5 
met6::URA3 DAY4a/al disrupted at met6 with URA3 diploid S. Dabrowski

 

 4 

 

 9,10\6 
RRY4 a ser1 ura3-52 trp1 leu2 his4 MET12 met13::CHIMERA1 met+ R.Raymond

 

 4 

 

6\8,9 
RRY5 a ser1 ura3-52 trp1 leu2 his4 $met12 met13::CHIMERA1 met+ R.Raymond

 

 4 

 

7\8,9 
RRY3/pVT-hMTHFR RRY3 with human MTHFR on pVT   R.Raymond

 

 4 

 

3\8,9 
RRY3/pVT-ecMTHFR RRY3 with E. coli MTHFR on pVT   R.Raymond

 

 4 

 

4\8,9 
RRY3/pVT-MET12 RRY3 with MET12 on pVT   R.Raymond

 

 4 

 

 5\8,9 
RRY3/pVT-MET13 RRY3 with MET13 on pVT   R.Raymond

 

 4 

 

9\7,8 
DAY4/pVT-MET13 DAY4 with MET13 on pVT   R.Raymond

 

 4 

 

 2\8,9 
DAY4/pVT-MET12 DAY4 with MET12 on pVT   R.Raymond 4 1\8,9
ATY1 a a ser1 ura3-52 trp1 leu2 his4 $ade16 ade+ ura- A. Tibbetts 5 1\1,2
ATY2 a a ser1 ura3-52 trp1 leu2 his4 ade17::URA3 ade+ ura+ A. Tibbetts 5 2\1,2
ATY3 a a ser1 ura3-52 trp1 leu2 his4 $ade16 ade17::URA3 ade- ura+ A. Tibbetts 5 3\1,2
BL21/H-ATIC2 E. coli expressing his-tagged ADE17 from pET vector   A. Tibbetts 5 4\1
ATY3/pH-ATIC1 his-tagged ADE16 in ATY3 ade- A. Tibbetts 5 5\1,2
ATY3/pH-ATIC2 his-tagged ADE17 in ATY3 ade+ A. Tibbetts 5 6\1,2
 D4/2 a ser1 ura3-52 trp1 leu2 his4 gly1::URA3 ura+ Y.Woldman 8 1\8
 D4/11 a ser1 ura3-52 trp1 leu2 his4 gly1::URA3 ura+ Y.Woldman 8 2\8 
 D4/9 a ser1 ura3-52 trp1 leu2 his4 gly1::URA3 ura+ Y.Woldman  8 3\8
 D4/16 a ser1 ura3-52 trp1 leu2 his4 gly1::URA3 ura+ Y.Woldman  8 4\8
 E2/19 a ser1 ura3-52 trp1 leu2 his4 shm2::LEU2 gly1::URA3 ura+leu+ Y.Woldman  8 5\8
 E2/27 a ser1 ura3-52 trp1 leu2 his4 shm2::LEU2 gly1::URA3 ura+leu+ Y.Woldman  8 6\8
 30/2/a al ser1 ura3-52 trp1 leu2 his4 gly1::URA3 ura+ Y.Woldman  8 1\9
 11/8/c a ser1 ura3-52 trp1 leu2 his4 gly1::URA3 ura+ Y.Woldman  8 2\9
 20/7/b a ser1 ura3-52 trp1 leu2 his4 gly1::URA3 ura+ Y.Woldman  8 4\9
 26/3/a a ser1 ura3-52 trp1 leu2 his4 gly1::URA3 ura+ Y.Woldman  8 5\9
 22/5/c ser1 URA3 trp1 leu2 his4 GLY1 ura+ Y.Woldman  8 3\9
 DAY3/MGCV a ser1 ura3-52 trp1 leu2 ade3-130 (his-) gcv::URA3 ade3 deletion; his- A.Bognar 8 6,7\9
MWY4.4$mis1 a ser1 ura3-52 trp1 leu2 his4 ade3-65 $mtd1 $mis1 D-N-(ade+ura-leu-)
(no plasmid)
C. Farmer 8 9\8
S2068B al thr4 ade1 trp1 GAL mal CUPres   YGSC 8 9,10\5
YWY9     Y.Woldman 8 1\5
YWY10     Y.Woldman 8 2\5
YWY11     Y.Woldman 8 3\5
YWY12     Y.Woldman 8 4\5
 Y342 al ura3-52 leu2 $spe2::LEU2 requires polyamines H. Tabor 8,9\9
 ATY2 a a ser1 ura3-52 trp1 leu2 his4 $ade17 ade+ ura- A. Tibbetts

 

 5 

 

1\3 
 ATY1.1 a a ser1 ura3-52 trp1 leu2 $ade16 HIS4 ade+ his+ A. Tibbetts

 

 5 

 

2\3,4 
 ATY2.1 a a ser1 ura3-52 trp1 leu2 ade17::URA3 HIS4 ade+ his+ ura- A. Tibbetts

 

 

3\3,4 
 ATY3.1 a a ser1 ura3-52 trp1 leu2 $ade16 ade17::URA3 HIS4 ade- his- ura+ A. Tibbetts

 

 

5\3,4 
 ATY3.1 a a ser1 ura3-52 trp1 leu2 $ade16 $ade17 HIS4 pHM53? ade- his- ura- leu+? A. Tibbetts

 

 

6\3,4 
 ATY1.2 a ser1 ura3-52 trp1 leu2 $ade16 c-mycADE17 HIS4 epitope-tagged ADE16 A. Tibbetts

 

 

7\2,3,4 
 ATY2.2 a a ser1 ura3-52 trp1 leu2 c-mycADE16 $ade17 epitope-tagged ADE17 A. Tibbetts

 

 

8\2,3,4 
 ATY1 al al ser1 ura3-52 trp1 leu2 his4 ade16::URA3 ade+ ura+ A. Tibbetts

 

 

9\3,4 
 ATY2 al al ser1 ura3-52 trp1 leu2 his4 ade17::URA3 ade+ ura+ A. Tibbetts

 

 

10\3,4 
 YPH500 al ura3-52 lys2-801 ade2-101 trp1-$63 his3-$200 leu2-$1 described in Sikorski and Heiter (1989) C. Barlowe

 

 

1\5 
 CBY73 al ura3-52 lys2-801 ade2-101 trp1-$63 his3-$200 leu2-$1 sly1::HIS3 with plasmid pXC5r (SLY1-myc LEU2 CEN) c-myc tagged Sly1p  C. Barlowe

 

 

2\5 
 CBY134 YPH501 a/al diploid with plasmid pXC6 (GAL1 SLY1-myc LEU2) c-myc Sly1p under GAL1 control  C. Barlowe

 

 

3\5 
 DAY4 al al ser1 ura3-52 trp1 leu2 his4   D. Appling

 

 

4\5 
 DAY4.1 (MWY5) a ser1 ura3-52 trp1 leu2 HIS4 HIS4+ DAY4 M. West

 

 

9\5,6 
 ATY1.1 al al ser1 ura3-52 trp1 leu2 $ade16 HIS4 ade+ his+ A. Tibbetts

 

 

5\5,6 
 ATY2.1 al al ser1 ura3-52 trp1 leu2 $ade17 HIS4 ade+ his+ A. Tibbetts

 

 

6\5,6 
 ATY4 a a ser1 ura3-52 trp1 leu2 c-mycADE16 ADE17 epitope-tagged ADE16 A. Tibbetts

 

 

7\5,6 
 ATY5 a a ser1 ura3-52 trp1 leu2 ADE16 c-mycADE17 epitope-tagged ADE17 A. Tibbetts

 

 

8\5,6 
 ATY2 a/al a/al ser1 ura3-52 trp1 leu2 $ade17 his4/HIS4 isogenic $ade16 A. Tibbetts

 

 

1,2\6 
 ATY1 a/al a/al ser1 ura3-52 trp1 leu2 $ade16 his4/HIS4 isogenic $ade17 A. Tibbetts

 

 

3,4\6 
 cdc25H al ura3-52 his3-200 ade2-101 lys2-801 trp-901 leu2-3 112 cdc25-2 Gal+ Cytotrap protein-protein interaction A. Tibbetts

 

 

10\6 
YCY1 ade2 ade3   C. Chang 6 6\2
YCY3 mis1 lys2 ade2 ade3   C. Chang 6 6\1
YCY4 a ade2 ADE3 shm2   C. Chang  6 1\1,2
YCY5 al ade2 ADE3 shm2   C. Chang 6 2\1,2
YCY6 a ade2 ade3 his3 ser1   C. Chang 6 3\1,2
YCY7  al ade2 ade3 ser1 his3   C. Chang 6 4\1,2
YCY38B al ade2 lys2-80 ura3-52 trp1   C. Chang    
WHY1a/al  a/al ura3-52 trp1 leu2 his4 ser1 $fau1 homozygous diploid fau1 disruptant W. Holmes 6 3\9,10
WHY1a a ura3-52 trp1 leu2 his4 ser1 $fau1   W. Holmes  6 4\9,10
WHY1al al ura3-52 trp1 leu2 his4 ser1 $fau1   W. Holmes  6 5,6\9,10
WHY2.1     W. Holmes 6 2\3,4
WHY3 a ura3-52 trp1 leu2 ser1 $ade16 $ade17 HIS4   W. Holmes 6  
WHY1.1 a ura3-52 trp1 leu2 ser1 $fau1 $ade16   W. Holmes  6  
WHY1.2 a ura3-52 trp1 leu2 ser1 $fau1 $ade17    W. Holmes  6  
WHY1.3 a ura3-52 trp1 leu2 ser1 $fau1 $ade16 $ade17    W. Holmes   6 6\3,4
WHY1.4 a ura3-52 trp1 leu2 his4 ser1 $fau1 $shm1   W. Holmes   6 1\5,6
WHY1.5 a ura3-52 trp1 leu2 his4 ser1 $fau1 $shm2   W. Holmes   6 2\5,6 
WHY1.6 a ura3-52 trp1 leu2 his4 ser1 $fau1 $shm1 $shm2   W. Holmes   6 3\5,6 
WHY1.7 a ura3-52 trp1 leu2 his4 ser1 $fau1 $met12   W. Holmes   6  
WHY1.8 a ura3-52 trp1 leu2 his4 ser1 $fau1 $met13   W. Holmes  6  
WHY1.9 a ura3-52 trp1 leu2 his4 ser1 $fau1 $met12 $met13   W. Holmes   6  
WHY1.3.1 a ura3-52 trp1 ser1 $fau1 $ade16 $ade17 ade2 HIS4   W. Holmes   6 5\5,6
WHY3.1 a ura3-52 trp1 ser1 $fau1 $ade16 $ade17 ade2 $his4   W. Holmes  6 7\3
CD208-2B al his3 leu2 ura3 trp1 fol3::HIS3 requires folate or folinic acid H. Cherest  6 9\3
CC933-1D     H. Cherest 6 10\3,4
WHY1a X CD208-2B   diploid W. Holmes 6 9\4
WHY1 X 3-5281   diploid   6 10\5
TR3 X ATY3.1   cross to produce WHY3 W. Holmes 6 5\1
38B16b       6 5\7
38B19d        6 5\8
38B7c       6 6\8
38B5b       6 7\8
38B1a       6 8\8
38B6c       6 9\7
38B1a X 38B4d       6 9\8
79B9b       6 6\7
79B12c       6 8\7
16B5a       6 7\7
TR2/pVT-ADE2       6 8\3
WHY3/pVT-FAU1       6 8\5,6
DAY4a/al fau1::URA3 diploid with heterozygous disruption of fau1 contains pHM53 W. Holmes 6 8\9,10
DAY4$mis1/pKS17     W. Holmes 6 4\5,6
DAY3al     W. Holmes 6 2\9,10
SCY1 a a ser1 leu2 his4 trp1 ura3-52 $met13   S. Chan SC2 1A,B
SCY1 al al ser1 leu2 his4 trp1 ura3-52 $met13   S. Chan SC2 1C, D
RRY2 a a ser1 leu2 his4 trp1 ura3-52 $met12   S. Chan SC2 1E,F
RRY2 al al ser1 leu2 his4 trp1 ura3-52 $met12   S. Chan SC2 1G,H
SCY3 a a ser1 leu2 his4 trp1 ura3-52 $met12 $met13   S. Chan SC2 1I,J
SCY3 al al ser1 leu2 his4 trp1 ura3-52 $met12 $met13   S. Chan SC2 2A,B
SCY4 a a ser1 leu2 his4 trp1 ura3-52 met13::CH1 chimera S. Chan SC2 2C,D
SCY4 al al ser1 leu2 his4 trp1 ura3-52 met13::CH1 chimera S. Chan SC2 2E,F
SCY5 a a ser1 leu2 his4 trp1 ura3-52 $met12 met13::CH1 chimera S. Chan SC2 2G,H
SCY5 al al ser1 leu2 his4 trp1 ura3-52 $met12 met13::CH1 chimera S. Chan SC2 2I,J
SCY6 a a ser1 leu2 his4 trp1 ura3-52 met13::AtMTHFR-1   S. Chan SC2 3A,B
SCY6 al al ser1 leu2 his4 trp1 ura3-52 met13::AtMTHFR-1   S. Chan SC2 3C,D
SCY7 a a ser1 leu2 his4 trp1 ura3-52 $met12 met13::AtMTHFR-1   S. Chan SC2 3E,F
SCY7 al al ser1 leu2 his4 trp1 ura3-52 $met12 met13::AtMTHFR-1   S. Chan SC2 3G,H
RRY1 al x RRY5 a diploid constructed to make SCY1, SCY3, SCY4, and SCY5   S. Chan SC2 3I,J
DAY4a/pVT101U DAY4a transformed with pVT101U   S. Chan SC2 4A
DAY4a/MET12-pVT101U DAY4a transformed with MET12-pVT101U   S. Chan SC2 4B
RRY2a/pVT101U RRY2a transformed with pVT101U   S. Chan SC2 4C
RRY2a/MET12-pVT101U RRY2a transformed with MET12-pVT101U   S. Chan SC2 4D
SCY5a/pVT101U SCY5a transformed with pVT101U   S. Chan SC2 4E
SCY5a/MET12-pVT101U SCY5a transformed with MET12-pVT101U   S. Chan SC2 4F
Y15305 al leu2 his3 ura3 lys2 ylr396c::KANMX4 vps33; Met+ EUROSCARF SC2 4G,H
DAY4vac (1) al ser1 leu2 HIS3 his4 trp1 ura3 ylr396c::KANMX4 vps33; Met+ S. Chan SC2 4I,J
DAY4vac (2) a ser1 leu2 HIS3 his4 trp1 ura3 ylr396c::KANMX4 vps33; Met+ S. Chan SC2 5A,B
RRY2vac a ser1 leu2 HIS3 his4 trp1 ura3 $met12 ylr396c::KANMX4 vps33; Met+ S. Chan SC2 5C,D
SCY4vac a ser1 leu2 HIS3 his4 trp1 ura3 met13::CH1 ylr396c::KANMX4 vps33; chimera; Met- on GF S. Chan SC2 5E,F
SCY6vac al ser1 leu2 his3 HIS4 trp1 ura3 met13::AtMTHFR-1 ylr396c::KANMX4 vps33; Met- on S and GF S. Chan SC2 5I,J
SCY7vac a ser1 leu2 HIS3 HIS4 trp1 ura3 $met12 met13::AtMTHFR-1 ylr396c::KANMX4 vps33; Met- on S and GF S. Chan SC2 6A,B
DAY4vac SER1 al leu2 his3 his4 trp1 ura3 ylr396c::KANMX4 vps33; Met+ S. Chan SC2 6C,D
RRY2vac SER1 a leu2 his3 HIS4 trp1 ura3 $met12 ylr396c::KANMX4 vps33; Met+ S. Chan SC2 6E,F
SCY4vac SER1 a leu2 his3 his4 trp1 ura3 met13::CH1 ylr396c::KANMX4 vps33; chimera; Met+ S. Chan SC2 6G,H
SCY6vac SER1 al leu2 his3 his4 trp1 ura3 met13::AtMTHFR-1 ylr396c::KANMX4 vps33; Met- on S and GF S. Chan SC2 6I,J
Y20212 a/al leu2/leu2 his3/his3 ura3/ura3 LYS2/lys2 MET15/met15 YER072w/yer072w::KANMX4 vtc1 heterozygous diploid EUROSCARF SC2 7A,B
Y10212 al leu2 his3 ura3 lys2 yer072w::KANMX4 vtc1 haploid EUROSCARF SC2 7C,D
DAY4pop a ser1 leu2 his4 trp1 ura3 yer072w::KANMX4 vtc1 S. Chan SC2 7E,F
SCY4pop (1) a ser1 leu2 his4 trp1 ura3 met13::CH1 yer072w::KANMX4 vtc1; chimera S. Chan SC2 7G,H
SCY4pop (2) a ser1 leu2 his4 trp1 ura3 met13::CH1 yer072w::KANMX4 vtc1; chimera S. Chan SC2 7I,J
DAY4a/c-Myc-MET12-pVT101U DAY4a transformed with c-Myc-MET12-pVT101U   S. Chan SC2 8A,B
SCY2.3 RRY2a transformed with c-Myc-MET12-pVT101U   S. Chan SC2 8C,D
DAY4a/c-Myc-MET12-pRS416 DAY4a transformed with c-Myc-MET12-pRS416   S. Chan SC2 8E,F
RRY2a/c-Myc-MET12-pRS416 RRY2a transformed with c-Myc-MET12-pRS416   S. Chan SC2 8G,H
DAY4cbs a ser1 leu2 his4 trp1 ura3-52 cys4::URA3 CBS mutant S. Chan SC2 8I,J
SCY4cbs a ser1 leu2 his4 trp1 ura3-52 met13::CH1 cys4::URA3 chimera; CBS mutant S. Chan SC2 9A,B
Y06696 a leu2 his3 ura3 met15 ygr155w::KANMX4 cys4; CBS mutant EUROSCARF SC2 9C,D
Y16696 al leu2 his3 ura3 lys2 ygr155w::KANMX4 cys4; CBS mutant EUROSCARF SC2 9E,F
Y20176 a/al leu2/leu2 his3/his3 ura3/ura3 LYS2/lys2 MET15/met15 YER043c/yer043c::KANMX4 diploid heterozygous sah1 EUROSCARF SC2 9G,H
sah1::KANMX4 (#5D) a leu2 his3 ura3 met15 yer043c::KANMX4 SAH mutant S. Chan SC2 10B,C
SCY3a/His-tagged MET13-pVT103U     S. Chan SC2 10F,G
DAY4 a/al dfr1::URA3 a/al ser1 leu2 his4 trp1 ura3-52 dfr1::URA3 dipolid S. Chan SC1 1A,B
DAY4 a/al dfr1::URA3 with pJM81 dfr1 mutant transformed with pJM81 containing TK dipolid S. Chan SC1 1C, D
DAY4 al with pJM81 obtained from dissection of DAY4 a/al dfr1::URA3 with pJM81   S. Chan SC1 1E
DAY4 a/al $dfr1 evicted URA3 cassette from dfr1 locus using pHM53   S. Chan SC1 1F,G
DAY4 a/al $dfr1 with TK-pVT101U dfr1 mutant transformed with TK-pVT101U dipolid S. Chan SC1 1H,I
DAY4 a/al $dfr1 with TK-pVT101U and DFR1-pRS315 dfr1 mutant with TK-pVT101U transformed with DFR1-pRS315 dipolid S. Chan SC1 2A,B
DAY4 al $dfr1 with TK-pVT101U and DFR1-pRS315 obtained from dissection of DAY4 a/al Ddfr1 with TK-pVT101U and DFR1-pRS315   S. Chan SC1 2C
DAY4 a a ser1 leu2 his4 trp1 ura3-52     SC1 2D,E
DAY4 al al ser1 leu2 his4 trp1 ura3-52     SC1 2F,G
Y22492 a/al leu2/leu2 his3/his3 ura3/ura3 MET15/met15 LYS2/lys2 YOR236W/yor236w::KANMX4  diploid heterozygote $dfr1 EUROSCARF SC1 2H,I
Y22492 with TK-pVT101U dfr1 mutant transformed with TK-pVT101U   S. Chan SC1 3A,B
Y22492 with TK-pVT101U and DFR1-pRS315 dfr1 mutant with TK-pVT101U transformed with DFR1-pRS315   S. Chan SC1 3C,D
Y22492 with TK-pVT101U #1 al leu2 his3 ura3 met15 LYS2 dfr1::KANMX4 with TK-pVT101U haploid segregant S. Chan SC1 3E,F
Y22492 with TK-pVT101U #15 a leu2 his3 ura3 MET15 lys2 dfr1::KANMX4 with TK-pVT101U haploid segregant S. Chan SC1 3G,H
Y22492 with TK-pVT101U #17 al leu2 his3 ura3 met15 lys2 dfr1::KANMX4 with TK-pVT101U haploid segregant S. Chan SC1 3I,J
Y22492 with TK-pVT101U #28 (SCY102) al leu2 his3 ura3 MET15 LYS2 dfr1::KANMX4 with TK-pVT101U haploid segregant; grows better on raffinose than galactose S. Chan SC1 4A,B
Y22492 with TK-pVT101U #37 a leu2 his3 ura3 MET15 lys2 dfr1::KANMX4 with TK-pVT101U haploid segregant S. Chan SC1 4C,D
Y22492 with TK-pVT101U #15 with DFR1-pRS315 #15 transformed with DFR1-pRS315 haploid segregant S. Chan SC1 4E,F
Y22492 with TK-pVT101U #2 (SCY101) al leu2 his3 ura3 MET15 LYS2 DFR1 with TK-pVT101U haploid segregant S. Chan SC1 4G,H
Y22492 with TK-pVT101U #4C a leu2 his3 ura3 MET15 LYS2 DFR1 with TK-pVT101U haploid segregant S. Chan SC1 4I,J
Y22492 with TK-pVT101U #28 with DFR1-pRS315 #28 transformed with DFR1-pRS315 haploid segregant S. Chan SC1 5A,B
SCY1001 $ifm1 $fmt1 with pVT101L   S. Chan SC1 7A,B
SCY1002 $ifm1 $fmt1 with IFM1 f.l.- pVT101L   S. Chan SC1 7C,D
SCY1003 $ifm1 $fmt1 with pre-G+C-IFM1-pVT101L   S. Chan SC1 7E,F
SCY4a$sam1 SCY4a sam1::KANMX4   S. Joslin SC1 6A
SCY4a$sam2 #1 SCY4a sam2::KANMX4   S. Joslin SC1 6B
SCY4a$sam2 #2 SCY4a sam2::KANMX4   S. Joslin SC1 6C
SCY4a$sam2 #3 SCY4a sam2::KANMX4   S. Joslin SC1 6D
           

 

Last Update: 22 June, 2004

Name: Dean Appling