The research in our laboratory focuses on group II intron and related bacterial and organellar reverse transcriptases (RTs), their reverse transcription mechanisms, association with CRISPR-Cas systems, and applications in high-throughput RNA sequencing (RNA-seq), including for the analysis of human cellular, exosomal, and plasma RNAs for RNA diagnostics and liquid biopsy of human diseases. We use a combination of genetic, biochemical, microbiological, and structural approaches. Methods employed include X-ray crystallography and cryo-EM, as well as high-throughput RNA and DNA sequencing and wide range of bioinformatic along with computational methods for analyzing sequence data and biomarker identification. Current research topics are described under the "Research" tab.