You've got a $#**% Ph.D.; you should know how to do this
There are some things I think everyone with a Ph.D. in Neuroscience should know how to do. This is directed mostly at people doing neurophysiology; someone else can write a sermon for those doing molecular biology. Here's the beginning of a list:
Electronics:
- You should be able to unpack a new piece of equipment, properly install it, and read the manual so you really understand how it works
- You should know how to check the calibration of your amplifier and how to recalibrate it if needed
- You should know what every knob and switch on your amplifier does and when to adjust it
- You should be able to look at a schematic and understand it
- You should know that the air vents on your equipment are there for a purpose and not cover them
- You should be able to properly solder two wires together, and how to change a component on a circuit board
- You should know to troubleshoot noise issues and check for proper grounding
Computers:
- You should know how to install updates to common software and the operating system
- You should know how to add memory or a new disk drive to your computer
- You should know how to back up your data, and do it often
- You should have more than a superficial knowledge of how your computer works
- You should be able to write a program in some language, or at least understand the concepts of loops, Boolean logic, and binary arithmetic
- You should know when it's appropriate to use a virus checker and make sure it's updated often
- You should know how to create a simple web page
- You should know how to connect to other computers in the lab when needed
- You should understand how email works and know how to set up the mail program on your computer
- You should know why using Webmail instead of a "real" email program is unwise
Data collection, analysis and presentation:
- You should understand sampling theory to select a reasonable sample rate and filtering corner frequency
- You should know how to select gains to use a reasonable proportion of the range of your ADC and DAC
- You should know what ADC and DAC mean and have a general idea of how they work
- You should understand how to calculate the scaling for ADC and DAC signals
- You should know how to make figures that can be read when projected (proper font size for axis legends, markers, labels, etc,)
- You should know that using K+ on a slide instead of K+ makes you look like an amateur
- You should know that showing a pixelated image shows that you don't understand resolution
Chemicals, drugs, and solutions
- You should know how to properly store chemicals (proper temperature, dessicated or not, etc)
- You should know how to measure out small volumes of chemicals and prevent contamination
- You should know what solvents to use for different drugs and how to assure they're really dissolved or suspended
- You should know how to properly buffer solutions, what acids or bases to use to adjust pH, and how to measure the osmolality of your solutions
- You should know how to calibrate the pH meters and osmometer
Tools and skills
- You should know how to choose the proper tool for a job
- You should know to put tools back when you're done with them (and don't mix up the metric and english allen wrenches)
- If you break a tool (or a drill bit) , stop by Breed's or Home Depot and get a replacement
- You should know about the different types of screws and bolts and when to use them
- You should know how to drill holes in metal, plastic, and wood
- You should know about drilling pilot holes
- You should know how to use a die to add threads to a rod or a tap to add threads to a hole
- You should know how to cut metal, plexiglass and wood
- You should know how to use the lathe and milling machine to make chambers or other items
Microscopes and micromanipulators
- You should know how to keep your microscope clean and properly adjusted
- You should know how to obtain Kohler illumination and adjust the differential contrast
- You should understand which optical filters are used under different circumstances
- You should know how to install your manipulators for maximum stability
General lab community
- You should accept that we work as a team and help each other as much as possible
- If you notice we're out of something simple (e.g., plastic containers for chemicals + dessicant), pick some up at the grocery store
- If you're pulling electrodes and notice the drawer holding electrode glass is in disarray, organize it; if you think drawer dividers would help, stop at Office Depot or the Container Store and get some
- If you think any area could be cleaned up or arranged better, take an hour and do it
- If email is sent to the whole lab asking for opinions, reply to the whole lab (or at the very minimum, reply to the sender)
- If you publish a paper, send a copy of the PDF file to everyone in the lab.