Publications

GOOGLE SCHOLAR PROFILE

https://scholar.google.com/citations?hl=en&user=zOIlcfsAAAAJ

 

2020

Bhattacharyya S., Walker, D.M. and Harshey, R.M. Dead cells release a 'necrosignal' that activates antibiotic survival pathways in bacterial swarming. Nature Comm. 11(1):4157. doi: 10.1038/s41467-020-17709-0.

Walker D.M. and Harshey R.M. Deep sequencing reveals new roles for MuB in transposition immunity and target-capture, and redefines the insular Ter region of E. coli  Mob DNA. 11:26. doi: 10.1186/s13100-020-00217-9.

Partridge, J.D., Nhu, N.T.Q., Dufour, Y. and Harshey, R.M. Tumble Suppression is a Conserved Feature of Swarming Motility. mBio. doi: 10.1128/mBio.01189-20

Partridge, J.D. and Harshey, R.M. Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Estalished Techniques in a series. J. Vis. Exp. doi: 10.3791/61364.

Walker, D.M., Freddolino, P.L. and Harshey, R.M. A Well-Mixed E. coli Genome: Widespread Contacts Revealed by Tracking Mu Transposition. Cell. doi: 10.1016/j.cell.2020.01.031

2019

Nieto, V., Partridge, J.D., Severin, G.B., Lai, R.Z., Waters, C.M., Parkinson, J.S. and Harshey, R.M. Under Elevated c-di-GMP in Escherichia coli, YcgR Alters Flagellar Motor Bias and Speed Sequentially, with Additional Negative Control of the Flagellar Regulon via the Adaptor Protein RssB. J. Bacteriol.  doi: 10.1128/JB.00578-19

Partridge, J.D., Nhu, N.T.Q., Dufour, Y.S., and Harshey, R.M. Escherichia coli remodels the chemotaxis pathway for swarming. mBio. Mar 10;2: doi: 10.1128/mBio.00316-19

2018

Bhattacharyya, S., Soniat, M.M., Walker, D., Jang, S., Finkelstein, I.J., and Harshey, R.M. Phage Mu Gam protein promotes NHEJ in concert with Escherichia coli ligase. Proc. Natl. Acad. Sci. Dec 11;115(50):  E11614-E11622. doi: 10.1073/pnas.1816606115

Partridge, J.D., Ariel, G., Schvartz, O., Harshey, R.M., and Be'er, A. The 2D architecture of a bacterial swarm has implications for antibiotic tolerance. Sci. Rep. Oct 25;8(1):  15823. doi: 10.1038/s41598-018-34192-2

2016

H. K. Kim and R. M. Harshey. A diguanylate cyclase acts as a cell division inhibitor in a two-step response to reductive and envelope stresses. MBio. Aug 9;7(4). pii: e00822-16. doi: 10.1128/mBio.00822-16

 

2015

G. Ariel, A. Rabani, S. Benisty, J. D. Partridge, R. M. Harshey and A. Be'er. Swarming Bacteria Migrate by Levy Walk. Nature Comm. Sep 25;6:8396. doi: 10.1038/ncomms9396
 

R. M. Harshey and J. D. Partridge. Shelter in a SwarmJ. Mol. Biol. Aug 12. pii: S0022-2836(15)00448-9. doi: 10.1016/j.jmb.2015.07.025
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S. Jang and R. M. Harshey. Repair of transposable phage Mu DNA insertions begins only when the E. coli replisome collides with the trasnpososome. Mol. Microbiol. Aug;97(4):746-58. doi: 10.1111/mmi.13061
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J. D. Partridge, V. Nieto and R. M. Harshey. A new player at the flagellar motor: FliL controls both motor output and bias. MBio. Feb 24;6(2):e02367. doi: 10.1128/mBio.02367-14
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J. Lee, A. F. Monzingo, A. T. Keatinge-Clay and R. M. Harshey. Structure of Salmonella FlhE, conserved member of a flagellar type III secretion operon. J. Mol. Biol. Mar;427:1254062 doi: 10.1016/j.jmb.2014.11.022
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2014 
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R.M. Harshey. Transposable Phage Mu. Microbiol. Spectr. Oct;2(5) doi: 10.1128/microbiolspec.MDNA3-0007-2014
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W. Choi, S. Jang and R. M. Harshey. Mu transpososome and RecBCD nuclease collaborate in the repair of simple Mu insertions. Proc. Natl. Acad. Sci. USA. Sep 30;111(39):14112-7. doi: 10.1073/pnas.1407562111
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W. Choi, R. P. Saha, S. Jang and R. M. Harshey. Controlling DNA degradation from a distance: a new role for the Mu transposition enhancer. Mol. Microbial. Nov;94(3):595-608. doi: 10.1111/mmi.12781
 
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2013

R. P. Saha, Z. Lou, L. Meng and R. M. Harshey. Transposable prophage Mu is organized as a stable chromosomal domain of E. coli. Plos Genetics. Nov;9(11):e1003902. doi: 10.1371/journal.pgen.1003902

J. D. Partridge and R. M. Harshey. Swarming: flexible roaming plans. J. Bacteriol. Mar;195(5):909-18. doi: 10.1128/JB.02063-12

J. D. Partridge and R. M. Harshey. More than motility: Salmonella flagella contribue to overriding friction and facilitating colony hydration during swarming. J. Bacteriol. Mar;195(5):919-29. doi: 10.1128/JB.02064-12
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2012

M. D. Lazova, M. T. Butler, T. S. Shimizu and R. M. Harshey. Salmonella chemoreceptors McpB and McpC mediate a repellent response to L-cystine: a potential mechanism to avoid oxidative conditions. Mol. Microbiol. May;84(4):697-711. doi: 10.1111/j.1365-2958.2012.08051.x
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Harhsey, R.M. The Mu story: how a maverick phage moved the field forward. Mob DNA. Dec 5;3(1):21. doi: 10.1186/1759-8753-3-21
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S. Jang. S. J. Sandler and R. M. Harshey.  Mu insertions are repaired by the double strand break repair pathway of E. coli. PLoS Genetics. Apr;8(4):e1002642.  doi: 10.1371/journal.pgen.1002642

J. Lee, and R. M. Harshey. Loss of FlhE in the flagellar type III secretion system allows proton inflx into Salmonella and E. coli. Mol Microbial. May;84(3):550-65. doi: 10.1111/j.1365-2958.2012.08043

2011

A. Be’er and R. M. Harshey. Collective motion of surfactant-producing bacteria imparts superdiffusivity to their upper surface. Biophys. J. 101:1017-24. doi: 10.1016/j.bpj.2011.07.019

J. Ge, Z. Lou, H. Cui, L. Shang and R. M. Harshey. Analysis of phage Mu DNA transposition by whole-genome E. coli tiling arrays reveals a complex relationship to distribution of large selection protein B, transcription and chromosome architectural elements. J. Biosci. Sep;36(4):587-601. PubMed PMID: 21857106
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2010

K. Paul. V. Nieto, W. C. Carliquist, D. F. Blair and R. M. Harshey. The c-di-GMP binding protein YcgR controls flagellar motor direction and speed to affect chemotaxis by a 'backstop break' mechanism. Mol. Cell. Apr 9;38(1):128-39. doi: 10.1016/j.molcel.2010.03.001

W. Choi and R. M. Harshey. DNA repair by the cryptic endonuclease activity of Mu transposase. Proc. Natl. Acad. Sci. USA. Jun 1;107(22):10014-9. doi: 10.1073/pnas.0912615107

 

M. Butler, Q. Wang and R. M. Harshey. Cell density and mobility protect swarming bacteria against antibiotic.Proc. Natl. Acad. Sci. USA. Feb 23;107(8):3776-81. doi: 10.1073/pnas.0910934107

R. P. Alexander, A. C. Lowenthal, R. M. Harshey and K. M. Ottemann. CheV: CheW-like coupling proteins at the core of the chemotaxis signaling network. Trends Microbial. Nov;18(11):494-503. doi: 10.1016/j.tim.2010.07.004
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J. Ge, Z. Lou and R. M. Harshey. Immunity of replicating Mu to self-integration: a novel mechanism employing MuB protein. Mob DNA. Feb 1;1(1):8. doi: 10.1186/1759-8753-1-8
 
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