Publications

#431 -Impact of internal fragments on top-down analysis of intact proteins by 193 nm UVPD

Dunham, S.D., Wi, B., Lantz, C., Loo, J.A., Brodbelt, J.S.
J. Proteome Res.
Volume 22, 2023. Pages 170-181.

#430 -Improving Ion Mobility Mass Spectrometry of Proteins through Tristate Gating and Optimization of Multiplexing Parameters

Butalewicz, J., Sanders, J.D., Clowers, B.H., Brodbelt, J.S.
JASMS
Volume 34, 2023. Pages 101-108.

#429 -A Deeper Understanding of Solvent-Based Ambient Ionization Mass Spectrometry: Are Molecular Profiles Primarily Dictated by Extraction Mechanisms?

Lin, M., Blevins, M.S., Sans, M.S., Brodbelt, J.S., Eberlin, L.S.
Anal. Chem.
Volume 42, 2022. Pages 14734-14744.

#428 -Advancing Orbitrap measurements of collision cross sections to multiple species for broad applications

James, V.K., Sanders, J.D,. Aizikov, K., Fort, K., Grinfeld, D., Makarov, A., Brodbelt, J.S.
Anal. Chem.
Volume 94, Issue 45, 2022. Pages 15613–15620.

#427 -Quantitative High Field NMR and Mass Spectrometry-Based Fatty Acid Sequencing Reveals Internal Structure in Ru-catalyzed Deuteration of Docoshexanoic Acid

Wang, D.H., Vidovic, D., McKay, A.I., Darwish, T., Park, H.G., Garza, S.M., Shields, S.W., Brodbelt, J.S., Wang, Z., Lacombe, R.J.S., Shmanai, Lysenko, I.L., V.V., Bekish, A.V., Schmidt, K., Redfield, C., Brenna, J.T., Shchepinov, M.S.
Anal. Chem.
Volume 94, 2022. Pages 12971-12980.

#426 -Uncommon Post-translational Modifications in Proteomics: ADP-Ribosylation, Tyrosine Nitration, and Tyrosine Sulfation

Bashyal, A., Brodbelt, J.S.
Mass Spec. Rev.
2022. Page 21811.

#425 -Structural characterization and quantitation of ether-linked glycerophospholipids in peroxisome biogenesis disorder tissue by ultraviolet photodissociation mass spectrometry

Blevins, M.S., Shields, S.W.J., Cui, W., Fallatah, W., Moser, A.B., Braverman, N.E., Brodbelt, J.S.
Anal. Chem.
Volume 94, 2022. Pages 12621-12629.

#424 -Nano-Hydrophobic Interaction Chromatography Coupled to Ultraviolet Photodissociation Mass Spectrometry for Analysis of Intact Proteins in Low Charge States

Blevins, M.S., Juetten, K.J., James, V., Butalewicz, J., Escobar, E.E., Lanzillotti, M., Sanders, J.D., Fort, K.L., Brodbelt, J.S.
J. Proteome Research
Volume 21, 2022. Pages 2493-2503.

#423 -Massively Parallel Selection of NanoCluster Beacons

Kuo, Y.A., Jung, C., Chen, Y.A., Kuo, H.C., Zhao, O.S., Nguyen, T.D., Rybarski, J.R., Hong, S., Chen, Y.I., Wylie, D.C., Hawkins, J.A., Walker, J.N., Shields, S.W.J., Brodbelt, J.S., Petty, J.T., Finkelstein, I.J., Yeh, H.-C.
Advanced Materials
Volume 34, Issue 41, 2022. Page 2204957.

#422 -Integrated Top-down and Bottom-up Mass Spectrometry for Characterization of Diselenide Bridging Patterns of Synthetic Selenoproteins

Watts, E., Thyer, R., Ellington, A., Brodbelt, J.S.
Anal. Chem.
Volume 94, Issue 32, 2022. Pages 11175-11184.

#421 -Enhancing the Signal-to-Noise of Diagnostic Fragment Ions of Unsaturated Glycerophospholipids via Precursor Exclusion Ultraviolet Photodissociation Mass Spectrometry (PEx-UVPD-MS)

Shields, S.W., Sanders, J.D., Brodbelt, J.S.
Anal. Chem.
Volume 94, Issue 32, 2022. Pages 11352-11359.

#420 -Deciphering Combinatorial Protein Post-translational Modifications by Top-Down Mass Spectrometry

Brodbelt, J.S.
Current Opin. Chem. Bio.
Volume 70, 2022. Page 102180

#419 -Investigation of Product Ions Generated by 193 nm Ultraviolet Photodissociation of Peptides and Proteins containing Disulfide Bonds

Macias, L.A., Brodbelt, J.S.
JASMS
Volume 33, Issue 7, 2022. Pages 1315–1324.

#418 -Symmetry of 4-Oxalocrotonate Tautomerase Trimers Influences Unfolding and Fragmentation in the Gas Phase

Sipe, S.N., Lancaster, E.B., Butalewicz, J.P., Whitman, C.P., Brodbelt, J.S.
JACS
Volume 144, Issue 27, 2022. Pages 12299–12309.

#417 -Exploring the Conformational and Binding Dynamics of HMGA2•DNA Complexes Using Trapped Ion Mobility Spectrometry – Mass Spectrometry

Fouque, K.J.D., Sipe, S.N., Garabedian, A., Mejia, G., Su, L., Leng, F., Hossen, Md.L., Chapagain, P., Brodbelt, J.S., Fernandez-Lima, F.
JASMS
Volume 33, Issue 7, 2022. Pages 1103-1112.

#416 -Separation and Collision Cross Section Measurements of Protein Complexes Afforded by a Modular Drift Tube Coupled to an Orbitrap Mass Spectrometer

Sipe, S.N., Sanders, J.D., Reinecke, T., Clowers, B.H., Brodbelt, J.S
Anal. Chem.
Volume 94, 2022. Pages 9434-9441.

#415 -Exploring the Conformations and Binding Location of HMGA2•DNA Complexes Using Ion Mobility Spectrometry and 193 nm Ultraviolet Photodissociation Mass Spectrometry

Sipe, S.N., Fouque, K.J.D., Garabedian, A., Leng, F., Fernandez-Lima, F., Brodbelt, J.S.
JASMS
Volume 33, Issue 7, 2022. Pages 1092-1102.

#414 -Mapping Paratopes of Nanobodies using Native Mass Spectrometry and Ultraviolet Photodissociation

Macias, L.A., Wang, X., Davies, B.W., Brodbelt, J.S.
Chem. Sci.
Volume 13, 2022. Pages 6610-6618.

#413 -Analysis of Viral Spike Protein N-glycosylation using Ultraviolet Photodissociation Mass Spectrometry

Escobar, E.E., Wang, S., Goswami, R., Lanzillotti, M., Li, L., McLellan, J.S., Brodbelt, J.S.
Anal. Chem.
Volume 94, issue 15 2022. Pages 5776-5784.

#412 -Ultraviolet Photodissociation and Activated Electron Photodetachment Mass Spectrometry for Top-Down Sequencing of Modified Oligoribonucleotides

Santos, I.C., Lanzillotti, M., Shilov, I., Basanta-Sanchez, M., Roushan, A., Lawler, R., Tang, W., Bern, M., Brodbelt, J.S.
JASMS
Volume 33, issue 3 2022. Pages 510-520.

#411 - Improving the center section sequence coverage of large proteins using stepped-fragment ion protection ultraviolet photodissociation

Dunham, S.D., Sanders, J.S., Holden, D.D., Brodbelt, J.S.
JASMS
Volume 33, issue 3,2022. Pages 446-456.

#410 - Enhanced Characterization of Cardiolipins via Hybrid 193 nm Ultraviolet Photodissociation Mass Spectrometry

Macias, L.A., Brodbelt, J.S..
Anal. Chem
Volume 94, 2022. Pages 3268-3277.

#409- Enhanced ion mobility separation and characterization of isomeric glycerophospholipids using absorption mode Fourier transform multiplexing and ultraviolet photodissociation mass spectrometry

Sanders, J.D., Shields, S.W., Escobar, E.E., Lanzillotti, M.B., Butalewicz, J.P., James, V.K., Blevins, M.S., Sipe, S.N., Brodbelt, J.S
Volume 22, 2022. Pages 4252-4259.

#408 - Influence of Primary Structure on Fragmentation of Native-Like Proteins by Ultraviolet Photodissociation

Macias, L.A., Sipe, S.N., Santos, I., Bashyal, A., Mehaffey, M.R., Brodbelt, J.S.
Volume 32, issue 12, 2021. Pages 2860-2873.

#407 - Characterizatoin of the T4gp32-ssDNA complexes by native, cross-linking, and ultraviolet photodissociation mass spectrometry

Blevins, M.S., Walker, J.N., Schaub, J.M., Finkelstein, I.J., Brodbelt, J.S.
Chem Sci.
Volume 12, 2021. Pages 13764-13776.

#406 - Characterization of HLA-A*02:01 MHC Immunopeptide Antigens Enhanced by Ultraviolet Photodissociation Mass Spectrometry

Watts, E., Potts, G.K., Ready, D.B., Thompson, A.M.G., Escobar, E.E., Patterson, M.J., Brodbelt, J.S.
Anal. Chem
Volume 93, 2021. Pages 13134-13142.

#405 - Relative Quantitation of Unsaturated Phosphatidylcholines using 193 nm Ultraviolet Photodissociation Parallel Reaction Monitoring Mass Spectrometry

Macias, L., Garza, K., Feider, C., Eberlin, L.S., Brodbelt, J.S.
Volume 143, issue 36, 2021. Pages 14622-14634.

#404 - Investigation of the Experimental Parameters of Ultraviolet Photodissociation for the Structural Characterization of Chondroitin Sulfate Glycosaminoglycan Isomers

Pepi, L.E, Leach, F.E., Klein, D.R., Brodbelt, J.S., Amster, I.J
Volume 32, 2021. Pages1759-1770.

#403 - Absorption Mode Fourier Transform Ion Mobility Mass Spectrometry Multiplexing Combined with Half-Window Apodization Windows Improves Resolution and Shortens Acquisition Times

Sanders, J.D., Butalewicz, J.P, Clowers, B.H., Brodbelt, J.S.
Anal. Chem
Volume 93, Issue 27, 2021. Pages 9513-9520.

#402 - What’s all the phos about? Insights into the phosphorylation state of the RNA polymerase II C-terminal domain via mass spectrometry

LeBlanc, B.M., Moreno, R.Y., Escobar, E., Ramani, M.K.V., Brodbelt, J.S., Zhang, Y.
RSC Chemical Biology

#401 - Directed evolution of an improved aminoacyl-tRNA synthetase for incorporation of L-3,4-dihydroxyphenylalanine (L-DOPA)

Thyer, R., d’Oelsnitz, S., Blevins, M.S., Klein, D.R., Brodbelt, J.S., Ellington, A.D.
Angew Chemie
Volume 60, 2021. Pages 14811-14816.

#400 - What’s all the phos about? Insights into the phosphorylation state of the RNA polymerase II C-terminal domain via mass spectrometry

LeBlanc, B.M., Moreno, R.Y., Escobar, E.E., Ramani, M.K.V., Brodbelt, J.S., Zhang, Y.
Royal Society of Chemistry
 

#399 - Evaluating Spatiotemporal Dynamics of Phosphorylation of RNA polymerase II Carboxy-terminal Domain Phosphorylation by Ultraviolet Photodissociation Mass Spectrometry

Escobar, E.E., Ramani, M.K.V., Zhang, Y.J., Brodbelt, J.S.
JACS
Volume 143, 2021. Pages 8488-8498.

#398 - Structural characterization of carbonic-anhydrase-arylsulfonamide complexes using ultraviolet photodissociation mass spectrometry

Santos, I.C., Brodbelt, J.S.
JASMS
Volume 32, 2021. Pages 1370-1379.

#397 - Characterization of isomers of Lipid A from Pseudomonas aeruginosa PAO1 by LC-MS/MS with HCD and UVPD

Bure, C., Senechal, C., Macias, L., Tokarski, C., Vilain, S., Brodbelt, J.S.
Analytical Chemistry
Volume 93, 2021. Pages 4255-4262.

#396 - Uniting Native Capillary Electrophoresis and Multistage Ultraviolet Photodissociation Mass Spectrometry for On-line Separation and Characterization of E. Coli Ribosomal Proteins and Protein Complexes

Mehaffey, M.R., Xia, J.Q., Brodbelt, J.S
Analytical Chemistry
Volume 92, 2021. Pages 15202-15211.

#395 - Structural basis for assembly of non-canonical small subunits into type I-C Cascade

O’Brien, R.E, Santos, I.C., Wrapp, D., Bravo, J.P.K, Schwartz, E.A., Brodbelt, J.S., Taylor, D.W.
Nature Comm.
Volume 11, 2021. Page 5931.

#394 - Recent developments in the characterization of nucleic acids by liquid chromatography, capillary electrophoresis, ion mobility, and mass spectrometry

Santos, I.C., Brodbelt, J.S.
J. Sep. Sci.
Volume 44, 2021. Pages 340-372.

#393 - Defining principles that influence antimicrobial peptide activity against capsulated Klebsiella pneumoniae

Fleeman, R.M., Macias, L.A., Brodbelt, J.S., Davies, B.W.
PNAS
Volume 117, 2020. Pages 27620-27626.

#392 - Interlaboratory Study for Characterizing Monoclonal Antibodies by Top-Down and Middle-Down Mass Spectrometry

) Srzentic, K., Fornelli, L., Tsybin, Y.O., Loo, J.A., Seckler, H., Agar, J.N., Anderson, L.C., Bai, D.L., Beck, A., Brodbelt, J.S., van der Burgt, Y., Chamot-Rooke, J., Chaterjee, S., Chen, Y., Clarke, D., Danis, P., Diedrich, J., D’Ippolito, R., Dupre, M., Gasilova, N., Ge, Y., Goo, Y., Goodlett, D., Gree, S., Haselmann, K., He, L., Hendrickson, C., Hinkle, J., Holt, M., Hughes, S., Hunt, D., Kelleher, N., Koshinov, A., Lin, Z., Malosse, C., Marshall, A., Menin, L., Millikin, R., Nagornov, K., Nioclardi, s., asa-Tolic, L., Pengelley, S. Quebbemann, N. Resemann, A., Sandoval, W., Sarin, R., Schmitte, N., Shabanowitz, J., Shaw, J., Shortreed, M., Smith, L., Sobott, F., Suckau, D., Toby, T., Weisbrod, C., Wildburger, N., Yates, J.R., Yoon, S., Young, N., Zhou, M.
JASMS
Volume 31, 2020. Pages 1783-1802.

#391 - Mapping a Conformational Epitope of Hemagglutinin A Using Native Mass Spectrometry and Ultraviolet Photodissociation

Mehaffey, M.R., Lee, J., Jun, J., Lanzillottti, M.B., Escobar, E.E., Morgenstern, K.R., Georgious, G., Brodbelt, J.S
Analytical Chemistry
Volume 92, 2020. Pages 11869-11878.

#390 - Elusive structural changes of New Delhi metallo-β-lactamase revealed by ultraviolet photodissociation mass spectrometry

Mehaffey, M.R., Ahn, Y.C., Rievera, D.D., Thomas, P.W., Cheng, Z., Crowder, MW., Thomas, P.W., Ahn, Y.-C., Pratt R.F., Fast, W., Brodbelt, J.S.,
Chem Sci.
Volume 11, 2020. Pages 8999-9010.

#389 - Comprehensive Middle-Down Mass Spectrometry Characterization of an Antibody-Drug Conjugate by Combined Ion Activation Methods

Watts, E., Williams, J.D., Miesbauer, L.J., Bruncko, M., Brodbelt, J.S.,
Analytical Chemistry
Volume 14, 2020. Pages 9790-9798.

#388 - Structural motifs for CTD kinase specificity on RNA polymerase II during eukaryotic transcription

Ramani, M.K.V., Escobar, E.E., Irani, S., Mayfield, J.E., Cotham, V.C., Xiao, H., Tadros, M., Brodbelt, J.S., Zhang, Y.J.,
ACS Chem Biol
Volume 15, 2020. Pages 2259-2272.

#387 - Unsaturation Elements and Other Modifications of Phospholipids in Bacteria: New Insight from Ultraviolet Photodissociation Mass Spectrometry

Blevins, M.S., James, V.K., Herrera, C.M., Purcell, A.B., Trent, M.S., Brodbelt, J.S.
Analytical Chemistry
Volume 92, 2020. Pages 9146-9155.

#386 - Double bond characterization of free fatty acids directly from biological tissues by ultraviolet photodissociation

Feider, C., Macias, L.A., Brodbelt, J.S., Eberlin, L.S.
Analytical Chemistry
Volume 92, 2020. Pages 8386-8395.

#385 - Localization of Double Bonds in Bacterial Glycerophospholipids Using 193 nm Ultraviolet Photodissociation in the Negative Mode

Klein, D.; Blevins, M.; Macias, L.; Douglas, M.; Trent, M.S.; Brodbelt, J.
Analytical Chemistry
Volume 92, 2020. Pages 5986-5993.

#384 - Precision Mapping of O-Linked N-Acetylglucosamine Sites in Proteins Using Ultraviolet Photodissociation Mass Spectrometry

Escobar, E., Vocadlo, D., Brodbelt, J.S
JACS
Volume 142, 2020. Pages 11569-11577.

#383 - Structural Evaluation of Protein/Metal Complexes via Native Electrospray Ultraviolet Photodissociation Mass Spectrometry

Crittenden, C.M., Novelli, E.T., Xu, G.N., Giles, D.H., Fies, W.A, Mehaffey, M.R., Dalby, K.N., Webb, L.J., Brodbelt, J.S.
JASMS
Volume 31, 2020. Pages 1140-1150.

#382 - Ion Activation for Peptides and Proteins

Macias, L, Santos, I., Brodbelt, J.S
Analytical Chemistry
Volume 92, 2020. Pages 227-251.

#381 - Footprints of nanoscale DNA-silver cluster chromophores via activated-electron photodetachment mass spectrometry

Blevins, MS., Kim, D., Crittenden, C.M., Hong, S., Yeh, H.C., Petty, J.T., Brodbelt, J.S.
ACS Nano
Volume 13, 2019. Pages 14070-14079.

#380 - Ultraviolet Photodissociation Mass Spectrometry for Analysis of Biological Molecules

Brodbelt, J.S., Morrison, L., Santos, I.
Chem Rev.
Volume 120, 2020. Pages 3328-3380.

#379 - Enhanced Sequence Coverage of Large Proteins by Combining Ultraviolet Photodissociation With Proton Transfer Reactions

Sanders, J.D., Mullen, C., Watts, E., Holden, D.D., Schwartz, J., Brodbelt, J.S.
Analytical Chemistry
Volume 92, 2020. Pages 1041-1049.

#378 - Enhanced Characterization of Membrane Protein Complexes by Ultraviolet Photodissociation Mass Spectrometry

Sipe, S.N., Patrick, J.W., Laganowsky, A., Brodbelt, J.S.
Analytical Chemistry
Volume 92, 2020. Pages 899-907.