About The Harshey Lab
The Harshey Lab studies two different areas. The first is the repair of Mu DNA transposition events. Transposition is not complete until short gaps created in the target on either side of the transposed element are repaired, yet how this happens is still not known. The repair assays the Harshey Lab is developing will be important for obtaining insights into this integral aspect of transposition, and should present a potent new target for drug development. Thus, knowledge of the repair process is not only necessary to fully understand the biology of transposition, but can be potentially used to block deleterious transposition events.
The second area Harshey researchers study is the flagellar motor as a sensor. In E. coli and Salmonella, chemosensory information from the environment is detected by chemoreceptors and transduced to the flagellar motor, modulating its CW/CCW bias, and enabling the bacterium to seek optimal habitats. Here, the motor is at the output end of the sensory response. Certain responses between the motor and bacterium play important roles in bacterial infection, surface colonization, persistence and pathogenesis. The Harshey Lab is currently elucidating this sensory mechanism. Lab scientists are also studying why swarming bacteria have a higher tolerance to antibiotics, and are using their knowledge of key motility mechanisms to design new antimicrobial targets.
2023 Research
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Bhattacharyya S, Bhattarai N, Pfannenstiel DM, Wilkins B, Singh A, Harshey RM. A heritable iron memory enables decision-making in Escherichia coli. Proc Natl Acad Sci U S A. 2023 Nov 28;120(48):e2309082120. Epub 2023 Nov 21. PMID: 37988472; PMCID: PMC10691332.
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Partridge JD, Dufour Y, Hwang Y, Harshey RM. Flagellar motor remodeling during swarming requires FliL. Mol Microbiol. 2023 Nov;120(5):670-683. Epub 2023 Sep 7. PMID: 37675594.
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Hwang Y, Harshey RM. A Second Role for the Second Messenger Cyclic-di-GMP in E. coli: Arresting Cell Growth by Altering Metabolic Flow. mBio. 2023 Apr 25;14(2):e0061923. Epub 2023 Apr 10. PMID: 37036337; PMCID: PMC10127611.
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Partridge JD, Harshey RM. Swarming Motility Assays in Salmonella. Methods Mol Biol. 2023;2646:147-158. PMID: 36842113.
2022 Research
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Bhattacharyya S, Bhattacharyya M, Pfannenstiel DM, Nandi AK, Hwang Y, Ho K, Harshey RM. Efflux-linked accelerated evolution of antibiotic resistance at a population edge. Mol Cell. 2022 Nov 17;82(22):4368-4385.e6. PMID: 36400010; PMCID: PMC9699456.
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Liou MJ, Miller BM, Litvak Y, Nguyen H, Natwick DE, Savage HP, Rixon JA, Mahan SP, Hiyoshi H, Rogers AWL, Velazquez EM, Butler BP, Collins SR, McSorley SJ, Harshey RM, Byndloss MX, Simon SI, Bäumler AJ. Host cells subdivide nutrient niches into discrete biogeographical microhabitats for gut microbes. Cell Host Microbe. 2022 Jun 8;30(6):836-847.e6. Epub 2022 May 13. PMID: 35568027; PMCID: PMC9187619.
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S Bhattacharyya, M Bhattacharyya, DM Pfannenstiel, AK Nandi, YS Hwang, K Ho and RM Harshey. Efflux-linked Accelerated Evolution of Antibiotic Resistance at a Population Edge. bioRxiv. January 1, 2022.
2021 Research:
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K Ho and RM Harshey. Goodbye PAM: Phage λ’s Red recombination system cripples PAMs and helps dodge CRISPR attacks. Cell Host & Microbe. October 13, 2021.
2020 Research:
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S Bhattacharyya, DM Walker, and RM Harshey. Dead cells release a 'necrosignal' that activates antibiotic survival pathways in bacterial swarming. Nature Comm. 11(1):4157. August 19, 2020.
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DM Walker and RM Harshey. Deep sequencing reveals new roles for MuB in transposition immunity and target-capture, and redefines the insular Ter region of E. coli. Mob DNA. 11:26. July 9, 2020.
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JD Partridge, NTQ Nhu, Y Dufour and RM Harshey. Tumble Suppression is a Conserved Feature of Swarming Motility. mBio.
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JD Partridge and RM Harshey. Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Estalished Techniques in a series. J. Vis. Exp. June 16, 2020.
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DM Walker, PL Freddolino and RM Harshey. A Well-Mixed E. coli Genome: Widespread Contacts Revealed by Tracking Mu Transposition. Cell. May 10, 2020.