Shigella species, which are closely related to Escherichia coli, can easily be maintained and stored in the laboratory. This article includes protocols for preparation of routine growth conditions and media, for storage of the bacteria, and for monitoring of the presence of the virulence plasmid.
Basic Protocol 1: Growth of S. flexneri from frozen stocks or agar stabs
Basic Protocol 2: Growth of S. flexneri in rich liquid medium
Alternate Protocol 1: Growth of S. flexneri in rich defined medium
Alternate Protocol 2: Growth of S. flexneri in minimal medium
Basic Protocol 3: Storage of S. flexneri in frozen stocks
Alternate Protocol 3: Storage of S. flexneri in agar stabs